PDB entry 4oo8

View 4oo8 on RCSB PDB site
Description: Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA
Class: Hydrolase/DNA/RNA
Keywords: Hydrolase-DNA-RNA complex
Deposited on 2014-01-31, released 2014-02-26
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-12-24, with a file datestamp of 2014-12-19.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: 0.224
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: crispr-associated endonuclease cas9/csn1
    Species: Streptococcus pyogenes serotype M1 [TaxId:301447]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99ZW2
      • engineered mutation (13)
      • engineered mutation (83)
      • engineered mutation (577)
      • engineered mutation (843)
    Domains in SCOPe 2.07: d4oo8a1, d4oo8a2, d4oo8a3, d4oo8a4
  • Chain 'B':
    Compound: RNA (97-mer)
  • Chain 'C':
    Compound: DNA (5'-d(*cp*cp*ap*gp*cp*cp*ap*ap*gp*cp*gp*cp*ap*cp*cp*tp*ap*ap*tp*tp*tp*cp*c)-3')
  • Chain 'D':
    Compound: crispr-associated endonuclease cas9/csn1
    Species: Streptococcus pyogenes serotype M1 [TaxId:301447]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99ZW2
      • engineered mutation (13)
      • engineered mutation (83)
      • engineered mutation (577)
    Domains in SCOPe 2.07: d4oo8d1, d4oo8d2, d4oo8d3
  • Chain 'E':
    Compound: RNA (97-mer)
  • Chain 'F':
    Compound: DNA (5'-d(*cp*cp*ap*gp*cp*cp*ap*ap*gp*cp*gp*cp*ap*cp*cp*tp*ap*ap*tp*tp*tp*cp*c)-3')
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4oo8A (A:)
gsghmdkkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsg
etaeatrlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherh
pifgnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnp
dnsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekkng
lfgnlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknl
sdaillsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqsk
ngyagyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihl
gelhailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpw
nfeevvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrk
paflsgeqkkaivdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhd
llkiikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrry
tgwgrlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsgq
gdslhehianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqkgqkn
srermkrieegikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdy
dvdaivpqsflkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrk
fdnltkaergglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvi
tlksklvsdfrkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvy
dvrkmiakseqeigkatakyffysnimnffkteitlangeirkrplietngetgeivwdk
grdfatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfds
ptvaysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdlii
klpkyslfelengrkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkq
lfveqhkhyldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftlt
nlgapaafkyfdttidrkrytstkevldatlihqsitglyetridlsqlggd

    Sequence, based on observed residues (ATOM records): (download) 
    >4oo8A (A:)
kkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgetaeat
rlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherhpifgni
vdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdnsdvd
klfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli
alslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsdaill
sdilrkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngyagyidggas
qeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgelhailrrqed
fypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfeevvdkgasa
qsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaflsgeqkkai
vdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhdllkiikdkdfld
neenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgwgrlsrklin
girdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvdslhehianlagspa
ikkgilqtvkvvdelvkvmgrhkpeniviemarenqtknsrermkrieegikelgsentq
lqneklylyylqngrdmyvdqeldinrlsdydvdaivpqsflkddsidnkvltrsdknrg
ksdnvpseevvkkmknywrqllnaklitqrkfdnltkaergglseldkagfikrqlvetr
qitkhvaqildsrmntirevkvitlksklvsdfrkdfqfykvreinnyhhahdaylnavv
gtalikkypklesefvygdykvyigkatakyffysnimnffktietngetgeivwdkgrd
fatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptv
aysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklp
kyslfelengrkrmlasagelqkgnelalpskyvnflylashypedneqkqlfveqhkhy
ldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlgapaafk
yfdttidrkrytstkevldatlihqsitglyetridlsqlg

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4oo8D (D:)
gsghmdkkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsg
etaeatrlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherh
pifgnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnp
dnsdvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekkng
lfgnlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknl
sdaillsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqsk
ngyagyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihl
gelhailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpw
nfeevvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrk
paflsgeqkkaivdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhd
llkiikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrry
tgwgrlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsgq
gdslhehianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqkgqkn
srermkrieegikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdy
dvdaivpqsflkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrk
fdnltkaergglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvi
tlksklvsdfrkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvy
dvrkmiakseqeigkatakyffysnimnffkteitlangeirkrplietngetgeivwdk
grdfatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfds
ptvaysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdlii
klpkyslfelengrkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkq
lfveqhkhyldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftlt
nlgapaafkyfdttidrkrytstkevldatlihqsitglyetridlsqlggd

    Sequence, based on observed residues (ATOM records): (download) 
    >4oo8D (D:)
kkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgetaeat
rlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherhpifgni
vdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdnsdvd
klfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli
alslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsdaill
sdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngyagy
idggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgelhai
lrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfeevv
dkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaflsg
eqkkaivdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhdllkiik
dkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgwgrl
srklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvdslhehian
lagspaikkgilqtvkvvdelvkvmgrhkpeniviemarrqlvetrqitkhvaqildsrm
ntkydendklirevkvitlksklvsdfrkdfqfykvreinnyhhahdaylnavvgtalik
kypklesefvygdfysnimnffklietngetgeivwdkgrdfatvrkvlsmpqvnivkkt
evqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptvaysvlvvakvekgkskklk
svkellgitimerssfeknpidfleakgykevkkdliiklpkyslfelengrkrmlasag
elqkgnelalpskyvnflylashyepedneqkqlfveqhkhyldeiieqisefskrvila
danldkvlsaynkhrdkpireqaeniihlftltnlgapaafkyfdttidrkrytstkevl
datlihqsitglyetridlsqlg

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.