PDB entry 6mwq

View 6mwq on RCSB PDB site
Description: Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP
Class: lyase/fluorescent protein
Keywords: synthetic construct, platform, single particle cryoEM, small protein display, LYASE-FLUORESCENT PROTEIN complex
Deposited on 2018-10-30, released 2018-11-21
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-01-08, with a file datestamp of 2020-01-03.
Experiment type: EM
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DARPin, Muscle-type aldolase chimeric fusion
    Species: Oryctolagus cuniculus [TaxId:9986]
    Gene: ALDOA
    Database cross-references and differences (RAF-indexed):
    • PDB 6MWQ (0-159)
    • Uniprot P00883 (160-492)
  • Chain 'B':
    Compound: DARPin, Muscle-type aldolase chimeric fusion
    Species: Oryctolagus cuniculus [TaxId:9986]
    Gene: ALDOA
    Database cross-references and differences (RAF-indexed):
    • PDB 6MWQ (0-159)
    • Uniprot P00883 (160-492)
  • Chain 'C':
    Compound: DARPin, Muscle-type aldolase chimeric fusion
    Species: Oryctolagus cuniculus [TaxId:9986]
    Gene: ALDOA
    Database cross-references and differences (RAF-indexed):
    • PDB 6MWQ (0-159)
    • Uniprot P00883 (160-492)
  • Chain 'D':
    Compound: DARPin, Muscle-type aldolase chimeric fusion
    Species: Oryctolagus cuniculus [TaxId:9986]
    Gene: ALDOA
    Database cross-references and differences (RAF-indexed):
    • PDB 6MWQ (0-159)
    • Uniprot P00883 (160-492)
  • Chain 'G':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (0-225)
      • conflict (62)
    Domains in SCOPe 2.08: d6mwqg_
  • Chain 'H':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6mwqh_
  • Chain 'I':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6mwqi_
  • Chain 'J':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6mwqj_

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6mwqG (G:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttlvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrie
lkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqq
ntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >6mwqH (H:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttlvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrie
lkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqq
ntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

    Sequence, based on observed residues (ATOM records): (download) 
    >6mwqH (H:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnriel
kgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqqn
tpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >6mwqI (I:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttlvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrie
lkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqq
ntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

    Sequence, based on observed residues (ATOM records): (download) 
    >6mwqI (I:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnriel
kgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqqn
tpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >6mwqJ (J:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttlvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnrie
lkgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqq
ntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

    Sequence, based on observed residues (ATOM records): (download) 
    >6mwqJ (J:)
skgeelftgvvpilveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlv
ttvqcfsrypdhmkqhdffksampegyvqertiffkddgnyktraevkfegdtlvnriel
kgidfkedgnilghkleynynshnvyimadkqkngikvnfkirhniedgsvqladhyqqn
tpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit