PDB entry 4kaf

View 4kaf on RCSB PDB site
Description: Crystal Structure of Haloalkane dehalogenase HaloTag7 at the resolution 1.5A, Northeast Structural Genomics Consortium (NESG) Target OR151
Class: hydrolase
Keywords: Structural Genomics, PSI-Biology, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG, HYDROLASE
Deposited on 2013-04-22, released 2013-06-05
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-06-05, with a file datestamp of 2013-05-31.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: 0.149
AEROSPACI score: 0.67 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: haloalkane dehalogenase
    Species: Rhodococcus sp. [TaxId:1831]
    Gene: dhaA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A3G3 (Start-302)
      • expression tag (11)
      • engineered mutation (56)
      • engineered mutation (67)
      • engineered mutation (87)
      • engineered mutation (96-97)
      • engineered mutation (132)
      • engineered mutation (137)
      • engineered mutation (164)
      • engineered mutation (169)
      • engineered mutation (176)
      • engineered mutation (181)
      • engineered mutation (184-185)
      • engineered mutation (204)
      • engineered mutation (233)
      • engineered mutation (236)
      • engineered mutation (266)
      • engineered mutation (273)
      • engineered mutation (281-282)
      • engineered mutation (300-301)
      • expression tag (303-306)
    Domains in SCOPe 2.08: d4kafa1, d4kafa2, d4kafa3
  • Chain 'B':
    Compound: haloalkane dehalogenase
    Species: Rhodococcus sp. [TaxId:1831]
    Gene: dhaA
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A3G3 (8-302)
      • expression tag (10)
      • expression tag (11)
      • engineered mutation (56)
      • engineered mutation (67)
      • engineered mutation (87)
      • engineered mutation (96-97)
      • engineered mutation (132)
      • engineered mutation (137)
      • engineered mutation (164)
      • engineered mutation (169)
      • engineered mutation (176)
      • engineered mutation (181)
      • engineered mutation (184-185)
      • engineered mutation (204)
      • engineered mutation (233)
      • engineered mutation (236)
      • engineered mutation (266)
      • engineered mutation (273)
      • engineered mutation (281-282)
      • engineered mutation (300-301)
      • expression tag (303-306)
      • expression tag (9)
    Domains in SCOPe 2.08: d4kafb1, d4kafb2
  • Heterogens: SCN, NA, EDO, PEG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4kafA (A:)
mghhhhhhshmaeigtgfpfdphyvevlgermhyvdvgprdgtpvlflhgnptssyvwrn
iiphvapthrciapdligmgksdkpdlgyffddhvrfmdafiealgleevvlvihdwgsa
lgfhwakrnperikgiafmefirpiptwdewpefaretfqafrttdvgrkliidqnvfie
gtlpmgvvrpltevemdhyrepflnpvdreplwrfpnelpiagepanivalveeymdwlh
qspvpkllfwgtpgvlippaeaarlakslpnckavdigpglnllqednpdligseiarwl
stleisg

    Sequence, based on observed residues (ATOM records): (download) 
    >4kafA (A:)
aeigtgfpfdphyvevlgermhyvdvgprdgtpvlflhgnptssyvwrniiphvapthrc
iapdligmgksdkpdlgyffddhvrfmdafiealgleevvlvihdwgsalgfhwakrnpe
rikgiafmefirpiptwdewpefaretfqafrttdvgrkliidqnvfiegtlpmgvvrpl
tevemdhyrepflnpvdreplwrfpnelpiagepanivalveeymdwlhqspvpkllfwg
tpgvlippaeaarlakslpnckavdigpglnllqednpdligseiarwlstleisg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4kafB (B:)
mghhhhhhshmaeigtgfpfdphyvevlgermhyvdvgprdgtpvlflhgnptssyvwrn
iiphvapthrciapdligmgksdkpdlgyffddhvrfmdafiealgleevvlvihdwgsa
lgfhwakrnperikgiafmefirpiptwdewpefaretfqafrttdvgrkliidqnvfie
gtlpmgvvrpltevemdhyrepflnpvdreplwrfpnelpiagepanivalveeymdwlh
qspvpkllfwgtpgvlippaeaarlakslpnckavdigpglnllqednpdligseiarwl
stleisg

    Sequence, based on observed residues (ATOM records): (download) 
    >4kafB (B:)
shmaeigtgfpfdphyvevlgermhyvdvgprdgtpvlflhgnptssyvwrniiphvapt
hrciapdligmgksdkpdlgyffddhvrfmdafiealgleevvlvihdwgsalgfhwakr
nperikgiafmefirpiptwdewpefaretfqafrttdvgrkliidqnvfiegtlpmgvv
rpltevemdhyrepflnpvdreplwrfpnelpiagepanivalveeymdwlhqspvpkll
fwgtpgvlippaeaarlakslpnckavdigpglnllqednpdligseiarwlstleisg