PDB entry 3v1m

View 3v1m on RCSB PDB site
Description: Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA
Class: hydrolase
Keywords: C-C bond hydrolase, Alpha/beta hydrolase fold, BPHD, ALPHA/BETA HYDROLASE, PCB DEGRADATION, META CLEAVAGE PRODUCT HYDROLASE, MCP HYDROLASE, 2-HYDROXY-6-OXO-6-PHENYL-HEXA-2,4-DIENOATE HYDROLASE, HYDROLASE
Deposited on 2011-12-09, released 2012-03-21
The last revision prior to the SCOPe 2.06 freeze date was dated 2012-04-11, with a file datestamp of 2012-04-06.
Experiment type: XRAY
Resolution: 1.92 Å
R-factor: 0.182
AEROSPACI score: 0.49 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    Species: BURKHOLDERIA XENOVORANS [TaxId:266265]
    Gene: bphD, Bxeno_C1120, Bxe_C1186
    Database cross-references and differences (RAF-indexed):
    • Uniprot P47229 (Start-285)
      • engineered mutation (111)
      • engineered mutation (264)
    Domains in SCOPe 2.06: d3v1ma_
  • Heterogens: MLI, HPK, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3v1mA (A:)
mtaltesstskfvkinekgfsdfnihyneagngetvimlhgggpgaggwsnyyrnvgpfv
dagyrvilkdspgfnksdavvmdeqrglvnaravkglmdaldidrahlvgnamggataln
faleypdrigklilmgpgglgpsmfapmpmegikllfklyaepsyetlkqmlqvflydqs
liteellqgrweaiqrqpehlknflisaqkaplstwdvtarlgeikaktfitwgrddrfv
pldhglkllwniddarlhvfskcgqwaqwehadefnrlvidflrha

    Sequence, based on observed residues (ATOM records): (download) 
    >3v1mA (A:)
ltesstskfvkinekgfsdfnihyneagngetvimlhgggpgaggwsnyyrnvgpfvdag
yrvilkdspgfnksdavvmdeqrglvnaravkglmdaldidrahlvgnamggatalnfal
eypdrigklilmgpgglgpsmfapmpmegikllfklyaepsyetlkqmlqvflydqslit
eellqgrweaiqrqpehlknflisaqkaplstwdvtarlgeikaktfitwgrddrfvpld
hglkllwniddarlhvfskcgqwaqwehadefnrlvidflrha