PDB entry 2wo5

View 2wo5 on RCSB PDB site
Description: structure of wild type e. coli n-acetylneuraminic acid lyase in space group p21 crystal form I
Class: lyase
Keywords: substrate specificity, carbohydrate metabolism, directed evolution, protein engineering, lyase, aldolase, schiff base
Deposited on 2009-07-21, released 2010-08-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2010-11-17, with a file datestamp of 2010-11-12.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.21424
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (5-7)
    Domains in SCOPe 2.08: d2wo5a1, d2wo5a2
  • Chain 'B':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2wo5b_
  • Chain 'C':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2wo5c_
  • Chain 'D':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2wo5d_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wo5A (A:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wo5A (A:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqir
rehpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wo5B (B:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wo5B (B:)
nlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereqvl
eivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdhyr
aiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirrehpd
lvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkvi
dlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2wo5C (C:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wo5C (C:)
atnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereq
vleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdh
yraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirreh
pdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnk
vidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2wo5D (D:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyq
meqirrehpdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wo5D (D:)
atnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereq
vleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdh
yraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalkqtsgdlyqmeqirreh
pdlvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnk
vidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqe