PDB entry 5axw

View 5axw on RCSB PDB site
Description: Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGGGT PAM)
Class: hydrolase/RNA/DNA
Keywords: CRISPR-Cas9, genome engineering, HYDROLASE-RNA-DNA complex
Deposited on 2015-08-01, released 2015-09-02
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-09-02, with a file datestamp of 2015-08-28.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas9
    Species: Staphylococcus aureus subsp. aureus [TaxId:46170]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • Uniprot J7RUA5
      • engineered mutation (582)
      • engineered mutation (948)
    Domains in SCOPe 2.07: d5axwa1, d5axwa2, d5axwa3, d5axwa4
  • Chain 'B':
    Compound: RNA (73-mer)
    Species: STAPHYLOCOCCUS AUREUS, synthetic [TaxId:1280]
  • Chain 'C':
    Compound: DNA (28-mer)
    Species: STAPHYLOCOCCUS AUREUS, synthetic [TaxId:1280]
  • Chain 'D':
    Compound: DNA (5'-d(*tp*tp*gp*gp*gp*tp*ap*g)-3')
    Species: STAPHYLOCOCCUS AUREUS, synthetic [TaxId:1280]
  • Heterogens: NA, PO4, EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5axwA (A:)
gshmkrnyilgldigitsvgygiidyetrdvidagvrlfkeanvennegrrskrgarrlk
rrrrhriqrvkkllfdynlltdhselsginpyearvkglsqklseeefsaallhlakrrg
vhnvneveedtgnelstkeqisrnskaleekyvaelqlerlkkdgevrgsinrfktsdyv
keakqllkvqkayhqldqsfidtyidlletrrtyyegpgegspfgwkdikewyemlmghc
tyfpeelrsvkyaynadlynalndlnnlvitrdenekleyyekfqiienvfkqkkkptlk
qiakeilvneedikgyrvtstgkpeftnlkvyhdikditarkeiienaelldqiakilti
yqssediqeeltnlnseltqeeieqisnlkgytgthnlslkainlildelwhtndnqiai
fnrlklvpkkvdlsqqkeipttlvddfilspvvkrsfiqsikvinaiikkyglpndiiie
lareknskdaqkminemqkrnrqtnerieeiirttgkenakyliekiklhdmqegkclys
leaipledllnnpfnyevdhiiprsvsfdnsfnnkvlvkqeeaskkgnrtpfqylsssds
kisyetfkkhilnlakgkgrisktkkeylleerdinrfsvqkdfinrnlvdtryatrglm
nllrsyfrvnnldvkvksinggftsflrrkwkfkkernkgykhhaedaliianadfifke
wkkldkakkvmenqmfeekqaesmpeieteqeykeifitphqikhikdfkdykyshrvdk
kpnrelindtlystrkddkgntlivnnlnglydkdndklkklinkspekllmyhhdpqty
qklklimeqygdeknplykyyeetgnyltkyskkdngpvikkikyygnklnahlditddy
pnsrnkvvklslkpyrfdvyldngvykfvtvknldvikkenyyevnskayeeakklkkis
nqaefiasfynndlikingelyrvigvnndllnrievnmidityreylenmndkrpprii
ktiasktqsikkystdilgnlyevkskkhpqiikkg

    Sequence, based on observed residues (ATOM records): (download) 
    >5axwA (A:)
rnyilgldigitsvgygiidyetrdvidagvrlfkeanvennegrrskrgarrlkrrrrh
riqrvkkllfdynlltdhselsginpyearvkglsqklseeefsaallhlakrrgvhnvn
eveedtgnelstkeqisrnskaleekyvaelqlerlkkdgevrgsinrfktsdyvkeakq
llkvqkayhqldqsfidtyidlletrrtyyegpgegspfgwkdikewyemlmghctyfpe
elrsvkyaynadlynalndlnnlvitrdenekleyyekfqiienvfkqkkkptlkqiake
ilvneedikgyrvtstgkpeftnlkvyhdikditarkeiienaelldqiakiltiyqsse
diqeeltnlnseltqeeieqisnlkgytgthnlslkainlildelwhtndnqiaifnrlk
lvpkkvdlsqqkeipttlvddfilspvvkrsfiqsikvinaiikkyglpndiiielarek
nskdaqkminemqkrnrqtnerieeiirttgkenakyliekiklhdmqegkclysleaip
ledllnnpfnyevdhiiprsvsfdnsfnnkvlvkqeeaskkgnrtpfqylsssdskisye
tfkkhilnlakgkgrisktkkeylleerdinrfsvqkdfinrnlvdtryatrglmnllrs
yfrvnnldvkvksinggftsflrrkwkfkkernkgykhhaedaliianadfifkewkkld
kakkvmenqmfmpeieteqeykeifitphqikhikdfkdykyshrvdkkpnrelindtly
strkddkgntlivnnlnglydkdndklkklinkspekllmyhhdpqtyqklklimeqygd
eknplykyyeetgnyltkyskkdngpvikkikyygnklnahlditddypnsrnkvvklsl
kpyrfdvyldngvykfvtvknldvikkenyyevnskayeeakklkkisnqaefiasfynn
dlikingelyrvigvnndllnrievnmidityreylenmndkrppriiktiasktqsikk
ystdilgnlyevkskkhpqiikk

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.