PDB entry 5a4f

View 5a4f on RCSB PDB site
Description: The mechanism of Hydrogen Activation by NiFe-hydrogenases.
Class: oxidoreductase
Keywords: oxidoreductase, nife-hydrogenase, hydrogen lysis, redox, fes cluster, catalysis
Deposited on 2015-06-09, released 2015-11-25
The last revision prior to the SCOPe 2.07 freeze date was dated 2016-01-13, with a file datestamp of 2016-01-08.
Experiment type: XRAY
Resolution: 1.25 Å
R-factor: N/A
AEROSPACI score: 0.62 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'L':
    Compound: Hydrogenase-1 large chain
    Species: ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 [TaxId:1403831]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0ACD8 (Start-581)
      • engineered mutation (117)
    Domains in SCOPe 2.07: d5a4fl_
  • Chain 'M':
    Compound: Hydrogenase-1 large chain
    Species: ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 [TaxId:1403831]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0ACD8 (Start-581)
      • engineered mutation (117)
    Domains in SCOPe 2.07: d5a4fm_
  • Chain 'S':
    Compound: Hydrogenase-1 small chain
    Species: ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 [TaxId:1403831]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d5a4fs_
  • Chain 'T':
    Compound: Hydrogenase-1 small chain
    Species: ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 [TaxId:1403831]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d5a4ft_
  • Heterogens: FCO, NI, MG, SO4, SF4, F3S, SF3, CL, HOH

PDB Chain Sequences:

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >5a4fL (L:)
mstqyetqgytinnagrrlvvdpitrieghmrcevnindqnvitnavscgtmfrgleiil
qgrdprdawafvericgvctgvhalasvyaiedaigikvpdnaniirnimlatlwchahl
vhfyqlagmdwidvldalkadprktselaqslsswpksspgyffdvqnrlkkfveggqlg
ifrngywghpqyklppeanlmgfahylealdfqreivkihavfggknphpnwivggmpca
inidesgavgavnmerlnlvqsiitrtadfinnvmipdalaigqfnkpwseigtglsdkc
vlsygafpdiandfgeksllmpggavingdfnnvlpvdlvdpqqvqefvdhawyrypndq
vgrhpfdgitdpwynpgdvkgsdtniqqlneqeryswikaprwrgnamevgplartliay
hkgdaatvesvdrmmsalnlplsgiqstlgrilcraheaqwaagklqyffdklmtnlkng
nlatastekwepatwptecrgvgfteaprgalghwaairdgkidlyqcvvpttwnasprd
pkgqigayeaalmntkmaipeqpleilrtlhsfdpclacsth

    Sequence, based on observed residues (ATOM records): (download) 
    >5a4fL (L:)
stqyetqgytinnagrrlvvdpitrieghmrcevnindqnvitnavscgtmfrgleiilq
grdprdawafvericgvctgvhalasvyaiedaigikvpdnaniirnimlatlwchahlv
hfyqlagmdwidvldalkadprktselaqslsswpksspgyffdvqnrlkkfveggqlgi
frngywghpqyklppeanlmgfahylealdfqreivkihavfggknphpnwivggmpcai
nidesgavgavnmerlnlvqsiitrtadfinnvmipdalaigqfnkpwseigtglsdkcv
lsygafpdiandfgeksllmpggavingdfnnvlpvdlvdpqqvqefvdhawyrypndqv
grhpfdgitdpwynpgdvkgsdtniqqlneqeryswikaprwrgnamevgplartliayh
kgdaatvesvdrmmsalnlplsgiqstlgrilcraheaqwaagklqyffdklmtnlkngn
latastekwepatwptecrgvgfteaprgalghwaairdgkidlyqcvvpttwnasprdp
kgqigayeaalmntkmaipeqpleilrtlhsfdpclacsth

  • Chain 'M':
    Sequence, based on SEQRES records: (download) 
    >5a4fM (M:)
mstqyetqgytinnagrrlvvdpitrieghmrcevnindqnvitnavscgtmfrgleiil
qgrdprdawafvericgvctgvhalasvyaiedaigikvpdnaniirnimlatlwchahl
vhfyqlagmdwidvldalkadprktselaqslsswpksspgyffdvqnrlkkfveggqlg
ifrngywghpqyklppeanlmgfahylealdfqreivkihavfggknphpnwivggmpca
inidesgavgavnmerlnlvqsiitrtadfinnvmipdalaigqfnkpwseigtglsdkc
vlsygafpdiandfgeksllmpggavingdfnnvlpvdlvdpqqvqefvdhawyrypndq
vgrhpfdgitdpwynpgdvkgsdtniqqlneqeryswikaprwrgnamevgplartliay
hkgdaatvesvdrmmsalnlplsgiqstlgrilcraheaqwaagklqyffdklmtnlkng
nlatastekwepatwptecrgvgfteaprgalghwaairdgkidlyqcvvpttwnasprd
pkgqigayeaalmntkmaipeqpleilrtlhsfdpclacsth

    Sequence, based on observed residues (ATOM records): (download) 
    >5a4fM (M:)
stqyetqgytinnagrrlvvdpitrieghmrcevnindqnvitnavscgtmfrgleiilq
grdprdawafvericgvctgvhalasvyaiedaigikvpdnaniirnimlatlwchahlv
hfyqlagmdwidvldalkadprktselaqslsswpksspgyffdvqnrlkkfveggqlgi
frngywghpqyklppeanlmgfahylealdfqreivkihavfggknphpnwivggmpcai
nidesgavgavnmerlnlvqsiitrtadfinnvmipdalaigqfnkpwseigtglsdkcv
lsygafpdiandfgeksllmpggavingdfnnvlpvdlvdpqqvqefvdhawyrypndqv
grhpfdgitdpwynpgdvkgsdtniqqlneqeryswikaprwrgnamevgplartliayh
kgdaatvesvdrmmsalnlplsgiqstlgrilcraheaqwaagklqyffdklmtnlkngn
latastekwepatwptecrgvgfteaprgalghwaairdgkidlyqcvvpttwnasprdp
kgqigayeaalmntkmaipeqpleilrtlhsfdpclacsth

  • Chain 'S':
    Sequence, based on SEQRES records: (download) 
    >5a4fS (S:)
lenkpripvvwihglectcctesfirsahplakdvilslisldyddtlmaaagtqaeevf
ediitqyngkyilavegnpplgeqgmfcissgrpfieklkraaagasaiiawgtcaswgc
vqaarpnptqatpidkvitdkpiikvpgcppipdvmsaiitymvtfdrlpdvdrmgrplm
fygqrihdkcyrrahfdagefvqswdddaarkgyclykmgckgpttynacsstrwndgvs
fpiqsghgclgcaengfwdrgsfysrvvdipqmgthstadtvgltalgvvaaavgvhava
savdqrrrhnqqptetehqpgnedkqarshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5a4fS (S:)
kpripvvwihglectcctesfirsahplakdvilslisldyddtlmaaagtqaeevfedi
itqyngkyilavegnpplgeqgmfcissgrpfieklkraaagasaiiawgtcaswgcvqa
arpnptqatpidkvitdkpiikvpgcppipdvmsaiitymvtfdrlpdvdrmgrplmfyg
qrihdkcyrrahfdagefvqswdddaarkgyclykmgckgpttynacsstrwndgvsfpi
qsghgclgcaengfwdrgsfysrv

  • Chain 'T':
    Sequence, based on SEQRES records: (download) 
    >5a4fT (T:)
lenkpripvvwihglectcctesfirsahplakdvilslisldyddtlmaaagtqaeevf
ediitqyngkyilavegnpplgeqgmfcissgrpfieklkraaagasaiiawgtcaswgc
vqaarpnptqatpidkvitdkpiikvpgcppipdvmsaiitymvtfdrlpdvdrmgrplm
fygqrihdkcyrrahfdagefvqswdddaarkgyclykmgckgpttynacsstrwndgvs
fpiqsghgclgcaengfwdrgsfysrvvdipqmgthstadtvgltalgvvaaavgvhava
savdqrrrhnqqptetehqpgnedkqarshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5a4fT (T:)
kpripvvwihglectcctesfirsahplakdvilslisldyddtlmaaagtqaeevfedi
itqyngkyilavegnpplgeqgmfcissgrpfieklkraaagasaiiawgtcaswgcvqa
arpnptqatpidkvitdkpiikvpgcppipdvmsaiitymvtfdrlpdvdrmgrplmfyg
qrihdkcyrrahfdagefvqswdddaarkgyclykmgckgpttynacsstrwndgvsfpi
qsghgclgcaengfwdrgsfysrv