PDB entry 6neg

View 6neg on RCSB PDB site
Description: N191D, F205S mutant of scoulerine-9-O methyltransferase from Thalictrum flavum complexed with S-ADENOSYL-L-HOMOCYSTEINE
Class: biosynthetic protein
Keywords: Scoulerine, Methyltransferase, Benzylisoquinoline Alkaloid, Plant natural products, BIOSYNTHETIC PROTEIN
Deposited on 2018-12-17, released 2019-12-18
The last revision prior to the SCOPe 2.07 freeze date was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: (S)-scoulerine 9-O-methyltransferase
    Species: Thalictrum flavum subsp. glaucum [TaxId:150095]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5C9L2
      • engineered mutation (210)
      • engineered mutation (224)
    Domains in SCOPe 2.07: d6nega1, d6nega2
  • Chain 'B':
    Compound: (S)-scoulerine 9-O-methyltransferase
    Species: Thalictrum flavum subsp. glaucum [TaxId:150095]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5C9L2
      • engineered mutation (210)
      • engineered mutation (224)
    Domains in SCOPe 2.07: d6negb1, d6negb2
  • Heterogens: SAH, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6negA (A:)
mgsshhhhhhssglvprgshmalqegvnylsglglsrliclpmalraaielnvfeiifqa
gpeaqlspaeivakiptknpnaaialdrilrmlgassilsvttmkdgrvyglteesrclv
adkngvsvvpmllftsdkavvesfynikdvvleegvipfdrthgmdffayagkeqsvnks
fnqamgagstiafdevfkvykgfhdlkelvdvgggigtslsniiskyphikginfelphv
iadapnypgvehiagnmfegvpnaqnillkwvlhdwddersikilqncwkalpeggtviv
vefvlpqilgnnaesfnaltpdllmmtlnpggkertttefdglakaagfaetkffpisqg
lhvmefhkatagvas

    Sequence, based on observed residues (ATOM records): (download) 
    >6negA (A:)
vnylsglglsrliclpmalraaielnvfeiifqagpeaqlspaeivakiptknpnaaial
drilrmlgassilsvttmkdgrvyglteesrclvadkngvsvvpmllftsdkavvesfyn
ikdvvleegvipfdrthgmdffayagkeqsvnksfnqamgagstiafdevfkvykgfhdl
kelvdvgggigtslsniiskyphikginfelphviadapnypgvehiagnmfegvpnaqn
illkwvlhdwddersikilqncwkalpeggtvivvefvlpqilgnnaesfnaltpdllmm
tlnpggkertttefdglakaagfaetkffpisqglhvmefhkata

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6negB (B:)
mgsshhhhhhssglvprgshmalqegvnylsglglsrliclpmalraaielnvfeiifqa
gpeaqlspaeivakiptknpnaaialdrilrmlgassilsvttmkdgrvyglteesrclv
adkngvsvvpmllftsdkavvesfynikdvvleegvipfdrthgmdffayagkeqsvnks
fnqamgagstiafdevfkvykgfhdlkelvdvgggigtslsniiskyphikginfelphv
iadapnypgvehiagnmfegvpnaqnillkwvlhdwddersikilqncwkalpeggtviv
vefvlpqilgnnaesfnaltpdllmmtlnpggkertttefdglakaagfaetkffpisqg
lhvmefhkatagvas

    Sequence, based on observed residues (ATOM records): (download) 
    >6negB (B:)
vnylsglglsrliclpmalraaielnvfeiifqagpeaqlspaeivakiptknpnaaial
drilrmlgassilsvttmkdgrvyglteesrclvadkngvsvvpmllftsdkavvesfyn
ikdvvleegvifdrthgmdffayagkeqsvnksfnqamgagstiafdevfkvykgfhdlk
elvdvgggigtslsniiskyphikginfelphviadapnypgvehiagnmfegvpnaqni
llkwvlhdwddersikilqncwkalpeggtvivvefvlpqilgnnaesfnaltpdllmmt
lnpggkertttefdglakaagfaetkffpisqglhvmefhkat