PDB entry 5ios

View 5ios on RCSB PDB site
Description: Flavin-dependent thymidylate synthase R90A variant in complex with FAD and deoxyuridine monophosphate
Class: transferase
Keywords: FAD-dependent, nucleotide biosynthesis, reductive methylation, TRANSFERASE
Deposited on 2016-03-08, released 2016-06-08
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-27, with a file datestamp of 2019-11-22.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [TaxId:243274]
    Gene: thyX, thy1, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (11)
      • engineered mutation (101)
    Domains in SCOPe 2.08: d5iosa1, d5iosa2
  • Chain 'B':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [TaxId:243274]
    Gene: thyX, thy1, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (101)
    Domains in SCOPe 2.08: d5iosb_
  • Chain 'C':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [TaxId:243274]
    Gene: thyX, thy1, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (101)
    Domains in SCOPe 2.08: d5iosc_
  • Chain 'D':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [TaxId:243274]
    Gene: thyX, thy1, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (101)
    Domains in SCOPe 2.08: d5iosd_
  • Heterogens: FAD, UMP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5iosA (A:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgaysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >5iosA (A:)
hmkidildkgfvelvdvmgndlsavraarvsflkdeerdrhlieylmkhghetpfehivf
tfhvkapifvarqwfrhriasynelsgaysklsyefyipsperlegykttippervteki
seivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwe
iqqyalaiarifkekcpwtfeaflkyaykgdilk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5iosB (B:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgaysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >5iosB (B:)
mkidildkgfvelvdvmgndlsavraarvsfdeerdrhlieylmkhghetpfehivftfh
vkapifvarqwfrhriasynelsgaysklsyefyipsperlegykttippervtekisei
vdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqweiqq
yalaiarifkekcpwtfeaflkyaykgdilkev

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5iosC (C:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgaysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >5iosC (C:)
mkidildkgfvelvdvmgndlsavraarvskdeerdrhlieylmkhghetpfehivftfh
vkapifvarqwfrhriasynelsgaysklsyefyipsperlegykttippervtekisei
vdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqweiqq
yalaiarifkekcpwtfeaflkyaykgdilkev

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5iosD (D:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgaysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >5iosD (D:)
mkidildkgfvelvdvmgndlsavraarvsfdmlkdeerdrhlieylmkhghetpfehiv
ftfhvkapifvarqwfrhriasynelsgaysklsyefyipsperlegykttippervtek
iseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqw
eiqqyalaiarifkekcpwtfeaflkyaykgdilkev