PDB entry 4l8w

View 4l8w on RCSB PDB site
Description: Crystal structure of gamma glutamyl hydrolase (H218N) from zebrafish complex with MTX polyglutamate
Class: Hydrolase
Keywords: Sandwiched-like domain, Hydrolase
Deposited on 2013-06-18, released 2014-05-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-05-14, with a file datestamp of 2014-05-09.
Experiment type: XRAY
Resolution: 2.39 Å
R-factor: 0.196
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'B':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8wb_
  • Chain 'D':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8wd_
  • Chain 'E':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8we_
  • Chain 'G':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8wg_
  • Chain 'I':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8wi_
  • Chain 'K':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (238)
    Domains in SCOPe 2.08: d4l8wk_
  • Heterogens: MTX, DGL, HOH

PDB Chain Sequences:

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4l8wB (B:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wB (B:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4l8wD (D:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wD (D:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4l8wE (E:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wE (E:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >4l8wG (G:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wG (G:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >4l8wI (I:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wI (I:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >4l8wK (K:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtclgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwnp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8wK (K:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtclgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwnpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn