PDB entry 4eo8

View 4eo8 on RCSB PDB site
Description: HCV NS5B polymerase inhibitors: Tri-substituted acylhydrazines as tertiary amide bioisosteres
Class: transferase/transferase inhibitor
Keywords: HCV, NS5B, RNA, RNA polymerase, polymerase inhibitor, Thumb Site 2 inhibitor, RNA-Dependent RNA Polymerase, TRANSFERASE-TRANSFERASE INHIBITOR complex
Deposited on 2012-04-13, released 2012-06-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2012-06-27, with a file datestamp of 2012-06-22.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.18
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: RNA-directed RNA polymerase
    Species: Hepatitis C virus (isolate BK) [TaxId:11105]
    Gene: NS5B
    Database cross-references and differences (RAF-indexed):
    • Uniprot P26663 (9-End)
      • expression tag (7-8)
      • engineered mutation (335)
      • engineered mutation (344)
      • engineered mutation (550)
    Domains in SCOPe 2.08: d4eo8a1, d4eo8a2
  • Chain 'B':
    Compound: RNA-directed RNA polymerase
    Species: Hepatitis C virus (isolate BK) [TaxId:11105]
    Gene: NS5B
    Database cross-references and differences (RAF-indexed):
    • Uniprot P26663 (9-End)
      • expression tag (7-8)
      • engineered mutation (335)
      • engineered mutation (344)
      • engineered mutation (550)
    Domains in SCOPe 2.08: d4eo8b1, d4eo8b2
  • Heterogens: 0S3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4eo8A (A:)
mashhhhhhsytwtgalitpcaaeesklpinalsnsllrhhnmvyattsrsaglrqkkvt
fdrlqvlddhyrdvlkemkakastvkakllsveeackltpphsakskfgygakdvrnlss
kavnhihsvwkdlledtvtpidttimaknevfcvqpekggrkparlivfpdlgvrvcekm
alydvvstlpqvvmgssygfqyspgqrveflvntwkskknpmgfsydtrcfdstvtendi
rveesiyqccdlapearqaikslterlyiggpltnskgqncgyrrcrasgvlttscgntl
tcylkasaacraaklqdctmlvngddlvvicesagvqedaaslrafteamtrysappgdp
pqpeydlelitscssnvsvahdasgkrvyyltrdpttplaraawetarhtpvnswlgnii
myaptlwarmilmthffsillaqeqlekaldcqiygacysiepldlpqiierlhglsafs
lhsyspgeinrvasclrklgvpplrvwrhrarsvrarllsqggraatcgkylfnwavktk
lkltpipaasqldlsgwfvagysggdiyhslsrarpr

    Sequence, based on observed residues (ATOM records): (download) 
    >4eo8A (A:)
hhsytwtgalitpcaaeesklpinalsnsllrhhnmvyattsrsaglrqkkvtfdrlqvl
ddhyrdvlkemkakastvkakllsveeackltpphsakskfgygakdvrnlsskavnhih
svwkdlledtvtpidttimaknevfcvqrkparlivfpdlgvrvcekmalydvvstlpqv
vmgssygfqyspgqrveflvntwkskknpmgfsydtrcfdstvtendirveesiyqccdl
apearqaikslterlyiggpltnskgqncgyrrcrasgvlttscgntltcylkasaacra
aklqdctmlvngddlvvicesagvqedaaslrafteamtrysappgdppqpeydlelits
cssnvsvahdasgkrvyyltrdpttplaraawetarhtpvnswlgniimyaptlwarmil
mthffsillaqeqlekaldcqiygacysiepldlpqiierlhglsafslhsyspgeinrv
asclrklgvpplrvwrhrarsvrarllsqggraatcgkylfnwavktklkltpipaasql
dlsgwfvagysggdiyh

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4eo8B (B:)
mashhhhhhsytwtgalitpcaaeesklpinalsnsllrhhnmvyattsrsaglrqkkvt
fdrlqvlddhyrdvlkemkakastvkakllsveeackltpphsakskfgygakdvrnlss
kavnhihsvwkdlledtvtpidttimaknevfcvqpekggrkparlivfpdlgvrvcekm
alydvvstlpqvvmgssygfqyspgqrveflvntwkskknpmgfsydtrcfdstvtendi
rveesiyqccdlapearqaikslterlyiggpltnskgqncgyrrcrasgvlttscgntl
tcylkasaacraaklqdctmlvngddlvvicesagvqedaaslrafteamtrysappgdp
pqpeydlelitscssnvsvahdasgkrvyyltrdpttplaraawetarhtpvnswlgnii
myaptlwarmilmthffsillaqeqlekaldcqiygacysiepldlpqiierlhglsafs
lhsyspgeinrvasclrklgvpplrvwrhrarsvrarllsqggraatcgkylfnwavktk
lkltpipaasqldlsgwfvagysggdiyhslsrarpr

    Sequence, based on observed residues (ATOM records): (download) 
    >4eo8B (B:)
hhsytwtgalitpcaaeesklpinalsnsllrhhnmvyattsrsaglrqkkvtfdrlqvl
ddhyrdvlkemkakastvkakllsveeackltpphsakskfgygakdvrnlsskavnhih
svwkdlledtvtpidttimaknevfcvqrkparlivfpdlgvrvcekmalydvvstlpqv
vmgssygfqyspgqrveflvntwkskknpmgfsydtrcfdstvtendirveesiyqccdl
apearqaikslterlyiggpltnskgqncgyrrcrasgvlttscgntltcylkasaacra
aklqdctmlvngddlvvicesagvqedaaslrafteamtrysappgdppqpeydlelits
cssnvsvahdasgkrvyyltrdpttplaraawetarhtpvnswlgniimyaptlwarmil
mthffsillaqeqlekaldcqiygacysiepldlpqiierlhglsafslhsyspgeinrv
asclrklgvpplrvwrhrarsvrarllsqggraatcgkylfnwavktklkltpipaasql
dlsgwfvagysggdiyhsls