PDB entry 3x3h

View 3x3h on RCSB PDB site
Description: Crystal Structure of the Manihot esculenta Hydroxynitrile Lyase (MeHNL) 3KP (K176P, K199P, K224P) triple mutant
Class: lyase
Keywords: Hydroxynitrilase, LYASE
Deposited on 2015-01-21, released 2016-03-02
The last revision prior to the SCOPe 2.06 freeze date was dated 2016-03-02, with a file datestamp of 2016-02-26.
Experiment type: XRAY
Resolution: 2.88 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3ha_
  • Chain 'B':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hb_
  • Chain 'C':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hc_
  • Chain 'D':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hd_
  • Chain 'E':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3he_
  • Chain 'F':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hf_
  • Chain 'G':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hg_
  • Chain 'H':
    Compound: (S)-hydroxynitrile lyase
    Species: Manihot esculenta [TaxId:3983]
    Gene: HNL
    Database cross-references and differences (RAF-indexed):
    • Uniprot P52705 (0-257)
      • engineered mutation (175)
      • engineered mutation (198)
      • engineered mutation (223)
    Domains in SCOPe 2.06: d3x3hh_

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hA (A:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hB (B:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hC (C:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hD (D:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hE (E:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hF (F:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hG (G:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3x3hH (H:)
mvtahfvlihtichgawiwhklkpaleraghkvtaldmaasgidprqieqinsfdeysep
lltfleklpqgekviivgescaglniaiaadryvdkiaagvfhnsllpdtvhspsytvek
llesfpdwrdteyftftnitgetittmklgfvllrenlftkctdgeyelakmvmrpgslf
qnvlaqrpkftekgygsipkvyiwtdqdkiflpdfqrwqianyppdkvyqvqggdhklql
tkteevahilqevadaya