PDB entry 3vyh

View 3vyh on RCSB PDB site
Description: Crystal structure of aW116R mutant of nitrile hydratase from Pseudonocardia thermophilla
Class: lyase
Keywords: nitrile hydratase, metalloenzyme, hydration, LYASE
Deposited on 2012-09-25, released 2013-11-13
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-11-13, with a file datestamp of 2013-11-08.
Experiment type: XRAY
Resolution: 1.63 Å
R-factor: 0.175
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Cobalt-containing nitrile hydratase subunit alpha
    Species: Pseudonocardia thermophila [TaxId:1848]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7SID2 (Start-203)
      • engineered mutation (115)
    Domains in SCOPe 2.08: d3vyha_
  • Chain 'B':
    Compound: Cobalt-containing nitrile hydratase subunit beta
    Species: Pseudonocardia thermophila [TaxId:1848]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3vyhb_
  • Heterogens: CO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3vyhA (A:)
mtenilrksdeeiqkeitarvkalesmlieqgilttsmidrmaeiyenevgphlgakvvv
kawtdpefkkrlladgteackelgigglqgedmmwventdevhhvvvctlcscyprpvlg
lppnwfkepqyrsrvvreprqllkeefgfevppskeikvwdsssemrfvvlpqrpagtdg
wseeelatlvtresmigvepakav

    Sequence, based on observed residues (ATOM records): (download) 
    >3vyhA (A:)
tenilrksdeeiqkeitarvkalesmlieqgilttsmidrmaeiyenevgphlgakvvvk
awtdpefkkrlladgteackelgigglqgedmmwventdevhhvvvctlcscyprpvlgl
ppnwfkepqyrsrvvreprqllkeefgfevppskeikvwdsssemrfvvlpqrpagtdgw
seeelatlvtresmigvepakav

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3vyhB (B:)
mngvydvggtdglgpinrpadepvfraewekvafamfpatfragfmgldefrfgieqmnp
aeylespyywhwirtyihhgvrtgkidleelerrtqyyrenpdaplpeheqkpeliefvn
qavygglpasrevdrppkfkegdvvrfstaspkgharraryvrgktgtvvkhhgayiypd
tagnglgecpehlytvrftaqelwgpegdpnssvyydcwepyielvdtkaaaa

    Sequence, based on observed residues (ATOM records): (download) 
    >3vyhB (B:)
mngvydvggtdglgpinrpadepvfraewekvafamfpatfragfmgldefrfgieqmnp
aeylespyywhwirtyihhgvrtgkidleelerrtqyyrenpdaplpeheqkpeliefvn
qavygglpasrevdrppkfkegdvvrfstaspkgharraryvrgktgtvvkhhgayiypd
tagnglgecpehlytvrftaqelwgpegdpnssvyydcwepyielv