PDB entry 2puh

View 2puh on RCSB PDB site
Description: Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA
Class: hydrolase
Keywords: C-C bond hydrolase, HYDROLASE
Deposited on 2007-05-09, released 2007-05-22
The last revision prior to the SCOPe 2.06 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 1.82 Å
R-factor: 0.17
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    Species: Burkholderia xenovorans [TaxId:36873]
    Gene: bphD
    Database cross-references and differences (RAF-indexed):
    • Uniprot P47229 (Start-285)
      • engineered (111)
    Domains in SCOPe 2.06: d2puha_
  • Heterogens: NA, MLI, HPK, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2puhA (A:)
mtaltesstskfvkinekgfsdfnihyneagngetvimlhgggpgaggwsnyyrnvgpfv
dagyrvilkdspgfnksdavvmdeqrglvnaravkglmdaldidrahlvgnamggataln
faleypdrigklilmgpgglgpsmfapmpmegikllfklyaepsyetlkqmlqvflydqs
liteellqgrweaiqrqpehlknflisaqkaplstwdvtarlgeikaktfitwgrddrfv
pldhglkllwniddarlhvfskcghwaqwehadefnrlvidflrha

    Sequence, based on observed residues (ATOM records): (download) 
    >2puhA (A:)
ltesstskfvkinekgfsdfnihyneagngetvimlhgggpgaggwsnyyrnvgpfvdag
yrvilkdspgfnksdavvmdeqrglvnaravkglmdaldidrahlvgnamggatalnfal
eypdrigklilmgpgglgpsmfapmpmegikllfklyaepsyetlkqmlqvflydqslit
eellqgrweaiqrqpehlknflisaqkaplstwdvtarlgeikaktfitwgrddrfvpld
hglkllwniddarlhvfskcghwaqwehadefnrlvidflrha