PDB entry 5syo

View 5syo on RCSB PDB site
Description: Crystal structure of a chimeric acetylcholine binding protein from Aplysia californica (Ac-AChBP) containing loop C from the human alpha 3 nicotinic acetylcholine receptor in complex with Cytisine
Class: acetylcholine-binding protein
Keywords: nicotinic, acetylcholine, AChBP, chimeric protein, ACETYLCHOLINE-BINDING PROTEIN
Deposited on 2016-08-11, released 2016-10-12
The last revision prior to the SCOPe 2.07 freeze date was dated 2016-10-12, with a file datestamp of 2016-10-07.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica, Homo sapiens [TaxId:6500, 9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (9-189)
      • expression tag (8)
      • engineered mutation (63)
    • Uniprot P32297 (190-205)
    • Uniprot Q8WSF8 (206-End)
      • engineered mutation (207)
    Domains in SCOPe 2.07: d5syoa1, d5syoa2
  • Chain 'B':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica, Homo sapiens [TaxId:6500, 9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (Start-189)
      • engineered mutation (63)
    • Uniprot P32297 (190-205)
    • Uniprot Q8WSF8 (206-End)
      • engineered mutation (207)
    Domains in SCOPe 2.07: d5syob_
  • Chain 'C':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica, Homo sapiens [TaxId:6500, 9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (Start-189)
      • engineered mutation (63)
    • Uniprot P32297 (190-205)
    • Uniprot Q8WSF8 (206-End)
      • engineered mutation (207)
    Domains in SCOPe 2.07: d5syoc_
  • Chain 'D':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica, Homo sapiens [TaxId:6500, 9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (Start-189)
      • engineered mutation (63)
    • Uniprot P32297 (190-205)
    • Uniprot Q8WSF8 (206-End)
      • engineered mutation (207)
    Domains in SCOPe 2.07: d5syod_
  • Chain 'E':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica, Homo sapiens [TaxId:6500, 9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (9-189)
      • expression tag (6-8)
      • engineered mutation (63)
    • Uniprot P32297 (190-205)
    • Uniprot Q8WSF8 (206-End)
      • engineered mutation (207)
    Domains in SCOPe 2.07: d5syoe1, d5syoe2
  • Heterogens: C5E, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5syoA (A:)
dykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevd
lvyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvth
dgsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyas
skyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >5syoA (A:)
lhsqanlmrlksdlfypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwklns
lmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrls
fmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqykh
dikyeeiypdvvlvvkfre

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5syoB (B:)
dykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevd
lvyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvth
dgsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyas
skyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >5syoB (B:)
sqanlmrlksdlfypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwklnslm
wdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrlsfm
cdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqykhdi
kyncceeiypdvvlvvkfre

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5syoC (C:)
dykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevd
lvyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvth
dgsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyas
skyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >5syoC (C:)
sqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwklnsl
mwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrlsf
mcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqykhd
ikyncceeiypdvvlvvkfrer

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5syoD (D:)
dykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevd
lvyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvth
dgsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyas
skyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >5syoD (D:)
sqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwklnsl
mwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrlsf
mcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqykhd
ikyncceeiypdvvlvvkfre

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >5syoE (E:)
dykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevd
lvyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvth
dgsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyas
skyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >5syoE (E:)
dklhsqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwk
lnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaq
rlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatq
ykhdikyncceeiypdvvlvvkfre