PDB entry 5ksw

View 5ksw on RCSB PDB site
Description: DHODB-I74D mutant
Class: oxidoreductase
Keywords: orotate, pyrimidine bisynthesis FeS cluster, electron transfer, dehydrogenase, reductase, protein engineering, OXIDOREDUCTASE
Deposited on 2016-07-10, released 2016-08-03
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-11-01, with a file datestamp of 2017-10-27.
Experiment type: XRAY
Resolution: 2.47 Å
R-factor: N/A
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: dihydroorotate dehydrogenase
    Species: LACTOCOCCUS LACTIS [TaxId:1358]
    Gene: pyrD, LG36_1039
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A089ZD72 (0-305)
      • conflict (62)
      • engineered mutation (69)
      • conflict (123)
      • conflict (182)
      • conflict (184)
      • conflict (303)
    Domains in SCOPe 2.07: d5kswa_
  • Chain 'B':
    Compound: Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
    Species: Lactococcus lactis subsp. lactis [TaxId:1360]
    Gene: pyrK, NCDO895_2366
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d5kswb1, d5kswb2
  • Chain 'C':
    Compound: dihydroorotate dehydrogenase
    Species: LACTOCOCCUS LACTIS [TaxId:1358]
    Gene: pyrD, LG36_1039
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A089ZD72 (0-End)
      • conflict (62)
      • engineered mutation (69)
      • conflict (123)
      • conflict (182)
      • conflict (184)
      • conflict (303)
    Domains in SCOPe 2.07: d5kswc_
  • Chain 'D':
    Compound: Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
    Species: Lactococcus lactis subsp. lactis [TaxId:1360]
    Gene: pyrK, NCDO895_2366
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d5kswd1, d5kswd2
  • Heterogens: FMN, CL, EDO, FAD, FES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5kswA (A:)
nrlsvklpgldlknpiipasgcfgfgeeyakyydlnklgsimvkattlhprfgnptprva
etasgmlnadglqnpglevimaeklpwlnenfpdlpiianvagseeddyvavcakigdap
nvkvielniscpnvkhggqafgtdpdvaaalvkackavskvplyvklspnvtdivpiaka
veaagadgltmintlmgvrfdlktrkpvlanitgglsgpaikpvalklihqvaqvvdipi
igmggvesaqdvlemymagasavavgtanfadpfvcpkiieklpevmdqygidslenliq
evknsk

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5kswB (B:)
mpklqemmtivsqrevasnifemvlkgelveemdlpgqflhlavpnasmllrrpisissw
dkvaktctilyrigdetsgtyeisklqsgakidvmgplgngfpvdevvstdkilivgggi
gvpplyelakqleekncqmtillgfasekvkilekefaelknvslkiatddgsygtkghv
gmlmeeidfevdalytcgapamlkavakkyeqlerlyismesrmacgigacyacvehdke
denhalkvcedgpvflgkqlll

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5kswC (C:)
nrlsvklpgldlknpiipasgcfgfgeeyakyydlnklgsimvkattlhprfgnptprva
etasgmlnadglqnpglevimaeklpwlnenfpdlpiianvagseeddyvavcakigdap
nvkvielniscpnvkhggqafgtdpdvaaalvkackavskvplyvklspnvtdivpiaka
veaagadgltmintlmgvrfdlktrkpvlanitgglsgpaikpvalklihqvaqvvdipi
igmggvesaqdvlemymagasavavgtanfadpfvcpkiieklpevmdqygidslenliq
evknsk

    Sequence, based on observed residues (ATOM records): (download) 
    >5kswC (C:)
nrlsvklpgldlknpiipasgcfgfgeeyakyydlnklgsimvkattlhprfgnptprva
etasgmlnadglqnpglevimaeklpwlnenfpdlpiianvagseeddyvavcakigdap
nvkvielniscpnvkhggqafgtdpdvaaalvkackavskvplyvklspnvtdivpiaka
veaagadgltmintlmgvrfdlktrkpvlanitgglsgpaikpvalklihqvaqvvdipi
igmggvesaqdvlemymagasavavgtanfadpfvcpkiieklpevmdqygidslenliq
evkns

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5kswD (D:)
mpklqemmtivsqrevasnifemvlkgelveemdlpgqflhlavpnasmllrrpisissw
dkvaktctilyrigdetsgtyeisklqsgakidvmgplgngfpvdevvstdkilivgggi
gvpplyelakqleekncqmtillgfasekvkilekefaelknvslkiatddgsygtkghv
gmlmeeidfevdalytcgapamlkavakkyeqlerlyismesrmacgigacyacvehdke
denhalkvcedgpvflgkqlll