PDB entry 4lem

View 4lem on RCSB PDB site
Description: Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis
Class: oxidoreductase
Keywords: Rossmann fold, dehydrogenase, oxidoreductase
Deposited on 2013-06-26, released 2014-05-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2015-03-04, with a file datestamp of 2015-02-27.
Experiment type: XRAY
Resolution: 2.27 Å
R-factor: N/A
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-End)
      • expression tag (19)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4lema1, d4lema2
  • Chain 'B':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-End)
      • expression tag (19)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4lemb1, d4lemb2
  • Chain 'C':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-562)
      • expression tag (19)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4lemc1, d4lemc2
  • Chain 'D':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-562)
      • expression tag (19)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4lemd1, d4lemd2
  • Chain 'E':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-End)
      • expression tag (19)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4leme1, d4leme2
  • Chain 'F':
    Compound: 1-pyrroline-5-carboxylate dehydrogenase
    Species: Mycobacterium tuberculosis [TaxId:83332]
    Gene: RVBD_1187
    Database cross-references and differences (RAF-indexed):
    • Uniprot O50443 (20-End)
      • engineered mutation (524)
    Domains in SCOPe 2.08: d4lemf_
  • Heterogens: B12, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4lemA (A:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemA (A:)
hmdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgerid
vvqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwr
ekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpl
dgfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvin
lvtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdf
vvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdi
tdlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddptd
esfvieyfgpllsvhvypderyeqildvidtgsryaltgaviaddrqavltaldrlrfaa
gnfyvndkptgavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyph
ma

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4lemB (B:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemB (B:)
hmdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgerid
vvqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwr
ekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpl
dgfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvin
lvtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdf
vvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdi
tdlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddptd
esfvieyfgpllsvhvypderyeqildvidtgsryaltgaviaddrqavltaldrlrfaa
gnfyvndkptgavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyph
mav

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4lemC (C:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemC (C:)
hmdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgerid
vvqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwr
ekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpl
dgfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvin
lvtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdf
vvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdi
tdlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddptd
esfvieyfgpllsvhvypderyeqildvidtgsryaltgaviaddrqavltaldrlrfaa
gnfyvndkptgavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyph
mavd

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4lemD (D:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemD (D:)
hmdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgerid
vvqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwr
ekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpl
dgfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvin
lvtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdf
vvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdi
tdlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddptd
esfvieyfgpllsvhvypderyeqildvidtgsryaltgaviaddrqavltaldrlrfaa
gnfyvndkptgavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyph
mavd

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4lemE (E:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemE (E:)
hmdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgerid
vvqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwr
ekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpl
dgfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvin
lvtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdf
vvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdi
tdlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddptd
esfvieyfgpllsvhvypderyeqildvidtgyaltgaviaddrqavltaldrlrfaagn
fyvndkptavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyphmav

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >4lemF (F:)
mgsshhhhhhssglvprgshmdaitqvpvpanepvhdyapkspertrlrtelasladhpi
dlphviggrhrmgdgeridvvqphrhaarlgtltnathadaaaaveaamsaksdwaalpf
deraavflraadllagpwrekiaaatmlgqsksvyqaeidavcelidfwrfnvafarqil
eqqpisgpgewnridyrpldgfvyaitpfnftsiagnlptapalmgntviwkpsitqtla
ayltmqlleaaglppgvinlvtgdgfavsdvaladprlagihftgstatfghlwqwvgtn
igryhsyprlvgetggkdfvvahasarpdvlrtalirgafdyqgqkcsavsrafiahsvw
qrmgdellakaaelrygditdlsnyggalidqrafvknvdaierakgaaavtvavggeyd
dsegyfvrptvllsddptdesfvieyfgpllsvhvypderyeqildvidtgsryaltgav
iaddrqavltaldrlrfaagnfyvndkptgavvgrqpfggargsdtndkagsplnllrwt
sarsiketfvaatdhiyphmavd

    Sequence, based on observed residues (ATOM records): (download) 
    >4lemF (F:)
mdaitqvpvpanepvhdyapkspertrlrtelasladhpidlphviggrhrmgdgeridv
vqphrhaarlgtltnathadaaaaveaamsaksdwaalpfderaavflraadllagpwre
kiaaatmlgqsksvyqaeidavcelidfwrfnvafarqileqqpisgpgewnridyrpld
gfvyaitpfnftsiagnlptapalmgntviwkpsitqtlaayltmqlleaaglppgvinl
vtgdgfavsdvaladprlagihftgstatfghlwqwvgtnigryhsyprlvgetggkdfv
vahasarpdvlrtalirgafdyqgqkcsavsrafiahsvwqrmgdellakaaelrygdit
dlsnyggalidqrafvknvdaierakgaaavtvavggeyddsegyfvrptvllsddpsfv
ieyfgpllsvhvypderyeqildvidtgsryaltgaviaddrqavltaldrlrfaagnfy
vndkptgavvgrqpfggargsdtndkagsplnllrwtsarsiketfvaatdhiyphma