PDB entry 3puw

View 3puw on RCSB PDB site
Description: Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-AlF4
Class: hydrolase/transport protein
Keywords: ATP Binding Cassette Nucleotide Binding Domain Substrate Binding Protein Transmembrane Domain, ABC Transporter importer ATPase, ATP binding Maltodextrin binding, transmembrane integral membrane, HYDROLASE-TRANSPORT PROTEIN complex
Deposited on 2010-12-06, released 2011-08-10
The last revision prior to the SCOPe 2.06 freeze date was dated 2011-09-28, with a file datestamp of 2011-09-23.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.222
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Maltose/maltodextrin import ATP-binding protein malK
    Species: Escherichia coli [TaxId:83333]
    Gene: b4035, ECDH10B_4224, JW3995, malK
    Database cross-references and differences (RAF-indexed):
    • Uniprot P68187 (Start-370)
      • expression tag (371)
    Domains in SCOPe 2.06: d3puwa1, d3puwa2, d3puwa3
  • Chain 'B':
    Compound: Maltose/maltodextrin import ATP-binding protein malK
    Species: Escherichia coli [TaxId:83333]
    Gene: b4035, ECDH10B_4224, JW3995, malK
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3puwb1, d3puwb2
  • Chain 'E':
    Compound: Maltose-binding periplasmic protein
    Species: Escherichia coli [TaxId:83333]
    Gene: b4034, ECDH10B_4223, JW3994, malE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (0-369)
      • expression tag (370-373)
    Domains in SCOPe 2.06: d3puwe1, d3puwe2
  • Chain 'F':
    Compound: Maltose transport system permease protein malF
    Species: Escherichia coli [TaxId:83333]
    Gene: b4033, ECDH10B_4222, JW3993, malF
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3puwf1, d3puwf2
  • Chain 'G':
    Compound: Maltose transport system permease protein malG
    Species: Escherichia coli [TaxId:83333]
    Gene: b4032, ECDH10B_4221, JW3992, malG
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3puwg_
  • Heterogens: MAL, PGV, UMQ, MG, ADP, ALF, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3puwA (A:)
masvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdl
figekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaev
lqlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlh
krlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmn
flpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvil
egevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgt
acrrlhkepgvasashhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puwA (A:)
asvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdlf
igekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaevl
qlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlhk
rlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmnf
lpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvile
gevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgta
crrlhkepgva

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3puwB (B:)
masvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdl
figekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaev
lqlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlh
krlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmn
flpvkvtataidqvqvelpmpnrqqvwlpvesrdvqvganmslgirpehllpsdiadvil
egevqvveqlgnetqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgt
acrrlhkepgvasashhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puwB (B:)
asvqlqnvtkawgevvvskdinldihegefvvfvgpsgcgkstllrmiagletitsgdlf
igekrmndtppaergvgmvfqsyalyphlsvaenmsfglklagakkevinqrvnqvaevl
qlahlldrkpkalsggqrqrvaigrtlvaepsvflldeplsnldaalrvqmrieisrlhk
rlgrtmiyvthdqveamtladkivvldagrvaqvgkplelyhypadrfvagfigspkmnf
lpvtataidqvqvelpmpnrqqvwlpvenmslgirpehllpsdiadvilegevqvveqlg
netqihiqipsirqnlvyrqndvvlveegatfaiglpperchlfredgtacrrlhkep

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3puwE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdea
lkdaqtritkasashhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >3puwE (E:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpdkafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyengkydikd
vgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtskv
nygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplg
avalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdea
lkdaqtritkasas

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3puwF (F:)
mdvikkkhwwqsdalkwsvlgllgllvgylvvlmyaqgeylfaittlilssaglyifanr
kayawryvypgmagmglfvlfplvctiaiaftnysstnqltferaqevlldrswqagkty
nfglypagdewqlalsdgetgknylsdafkfggeqklqlkettaqpegeranlrvitqnr
qalsditailpdgnkvmmsslrqfsgtqplytldgdgtltnnqsgvkyrpnnqigfyqsi
tadgnwgdeklspgytvttgwknftrvftdegiqkpflaifvwtvvfslitvfltvavgm
vlaclvqwealrgkavyrvllilpyavpsfisilifkglfnqsfgeinmmlsalfgvkpa
wfsdpttartmliivntwlgypymmilcmgllkaipddlyeasamdgagpfqnffkitlp
llikpltplmiasfafnfnnfvliqlltnggpdrlgtttpagytdllvnytyriafeggg
gqdfglaaaiatlifllvgalaivnlkatrmkfd

    Sequence, based on observed residues (ATOM records): (download) 
    >3puwF (F:)
wqsdalkwsvlgllgllvgylvvlmyaqgeylfaittlilssaglyifanrkayawryvy
pgmagmglfvlfplvctiaiaftnysstnqltferaqevlldrswqagktynfglypagd
ewqlalsdgetgknylsdafkfggeqklqlkettaqpegeranlrvitqnrqalsditai
lpdgnkvmmsslrqfsgtqplytldgdgtltnnqsgvkyrpnnqigfyqsinwgdeklsp
gytvttgwknftrvftdegiqkpflaifvwtvvfslitvfltvavgmvlaclvqwealrg
kavyrvllilpyavpsfisilifkglfnqsfgeinmmlsalfgvkpawfsdpttartmli
ivntwlgypymmilcmgllkaipddlyeasamdgagpfqnffkitlpllikpltplmias
fafnfnnfvliqlltnggpdrlgtttpagytdllvnytyriafeggggqdfglaaaiatl
ifllvgalai

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3puwG (G:)
mamvqpksqkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwk
lalgfsveqadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkat
llkgmlifqmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgy
fetidssleeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdv
nsytlavgmqqylnpqnylwgdfaaaavmsalpitivfllaqrwlvngltaggvkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3puwG (G:)
qkarlfithlllllfiaaimfpllmvvaislrqgnfatgslipeqiswdhwklalgfsve
qadgritpppfpvllwlwnsvkvagisaigivalsttcayafarmrfpgkatllkgmlif
qmfpavlslvalyalfdrlgeyipfiglnthggvifaylggialhvwtikgyfetidssl
eeaaaldgatpwqafrlvllplsvpilavvfilsfiaaitevpvaslllrdvnsytlavg
mqqylnpqnylwgdfaaaavmsalpitivfllaqrwlvngltaggvkg