PDB entry 2pyj

View 2pyj on RCSB PDB site
Description: Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Class: replication, transferase/DNA
Keywords: protein-dna complex, REPLICATION, TRANSFERASE-DNA COMPLEX
Deposited on 2007-05-16, released 2007-07-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-04-15, with a file datestamp of 2020-04-10.
Experiment type: XRAY
Resolution: 2.03 Å
R-factor: N/A
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase
    Species: BACILLUS PHAGE PHI29 [TaxId:10756]
    Gene: 2, gp2
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03680 (Start-574)
      • engineered (11)
      • engineered (65)
    Domains in SCOPe 2.08: d2pyja1, d2pyja2
  • Chain 'B':
    Compound: DNA polymerase
    Species: BACILLUS PHAGE PHI29 [TaxId:10756]
    Gene: 2, gp2
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03680 (Start-574)
      • engineered (11)
      • engineered (65)
    Domains in SCOPe 2.08: d2pyjb1, d2pyjb2
  • Chain 'J':
    Compound: 5'-d(GACTGCTTA(DOC)-3'
  • Chain 'K':
    Compound: 5'-d(ACACGTAAGCAGTC)-3'
  • Chain 'Q':
    Compound: 5'-d(GACTGCTTA(DOC)-3'
  • Chain 'R':
    Compound: 5'-d(ACACGTAAGCAGTC)-3'
  • Chain 'X':
    Compound: 5'-d(GACTGCTTA(DOC)-3'
  • Chain 'Y':
    Compound: 5'-d(ACACGTAAGCAGTC)-3'
  • Heterogens: EDO, MN, MG, DGT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2pyjA (A:)
    mkhmprkmyscafetttkvedcrvwaygymniedhseykignsldefmawvlkvqadlyf
    hnlkfagafiinwlerngfkwsadglpntyntiisrmgqwymidiclgykgkrkihtviy
    dslkklpfpvkkiakdfkltvlkgdidyhkerpvgykitpeeyayikndiqiiaealliq
    fkqgldrmtagsdslkgfkdiittkkfkkvfptlslgldkevryayrggftwlndrfkek
    eigegmvfdvnslypaqmysrllpygepivfegkyvwdedyplhiqhircefelkegyip
    tiqikrsrfykgneylkssggeiadlwlsnvdlelmkehydlynveyisglkfkattglf
    kdfidkwtyikttsegaikqlaklmlnslygkfasnpdvtgkvpylkengalgfrlgeee
    tkdpvytpmgvfitawaryttitaaqacydriiycdtdsihltgteipdvikdivdpkkl
    gywahestfkrakylrqktyiqdiymkevdgklvegspddytdikfsvkcagmtdkikke
    vtfenfkvgfsrkmkpkpvqvpggvvlvddtftik
    

    Sequence, based on observed residues (ATOM records): (download)
    >2pyjA (A:)
    prkmyscafetttkvedcrvwaygymniedhseykignsldefmawvlkvqadlyfhnlk
    fagafiinwlerngfkwsadglpntyntiisrmgqwymidiclgykgkrkihtviydslk
    klpfpvkkiakdfkltvlkgdidyhkerpvgykitpeeyayikndiqiiaealliqfkqg
    ldrmtagsdslkgfkdiittkkfkkvfptlslgldkevryayrggftwlndrfkekeige
    gmvfdvnslypaqmysrllpygepivfegkyvwdedyplhiqhircefelkegyiptiqi
    krsrfykgneylkssggeiadlwlsnvdlelmkehydlynveyisglkfkattglfkdfi
    dkwtyikttsegaikqlaklmlnslygkfasnpdvtgkvpylkengalgfrlgeeetkdp
    vytpmgvfitawaryttitaaqacydriiycdtdsihltgteipdvikdivdpkklgywa
    hestfkrakylrqktyiqdiymkevvegspddytdikfsvkcagmtdkikkevtfenfkv
    gfsrkmkpkpvqvpggvvlvddtftik
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >2pyjB (B:)
    mkhmprkmyscafetttkvedcrvwaygymniedhseykignsldefmawvlkvqadlyf
    hnlkfagafiinwlerngfkwsadglpntyntiisrmgqwymidiclgykgkrkihtviy
    dslkklpfpvkkiakdfkltvlkgdidyhkerpvgykitpeeyayikndiqiiaealliq
    fkqgldrmtagsdslkgfkdiittkkfkkvfptlslgldkevryayrggftwlndrfkek
    eigegmvfdvnslypaqmysrllpygepivfegkyvwdedyplhiqhircefelkegyip
    tiqikrsrfykgneylkssggeiadlwlsnvdlelmkehydlynveyisglkfkattglf
    kdfidkwtyikttsegaikqlaklmlnslygkfasnpdvtgkvpylkengalgfrlgeee
    tkdpvytpmgvfitawaryttitaaqacydriiycdtdsihltgteipdvikdivdpkkl
    gywahestfkrakylrqktyiqdiymkevdgklvegspddytdikfsvkcagmtdkikke
    vtfenfkvgfsrkmkpkpvqvpggvvlvddtftik
    

    Sequence, based on observed residues (ATOM records): (download)
    >2pyjB (B:)
    hmprkmyscafetttkvedcrvwaygymniedhseykignsldefmawvlkvqadlyfhn
    lkfagafiinwlerngfkwsadglpntyntiisrmgqwymidiclgykgkrkihtviyds
    lkklpfpvkkiakdfkltvlkgdidyhkerpvgykitpeeyayikndiqiiaealliqfk
    qgldrmtagsdslkgfkdiittkkfkkvfptlslgldkevryayrggftwlndrfkekei
    gegmvfdvnslypaqmysrllpygepivfegkyvwdedyplhiqhircefelkegyipti
    qikrsrfykgneylkssggeiadlwlsnvdlelmkehydlynveyisglkfkattglfkd
    fidkwtyikttsegaikqlaklmlnslygkfasnpdvtgkvpylkengalgfrlgeeetk
    dpvytpmgvfitawaryttitaaqacydriiycdtdsihltgteipdvikdivdpkklgy
    wahestfkrakylrqktyiqdiymkevgklvegspddytdikfsvkcagmtdkikkevtf
    enfkvgfsrkmkpkpvqvpggvvlvddtftik
    

  • Chain 'J':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'Q':
    No sequence available.

  • Chain 'R':
    No sequence available.

  • Chain 'X':
    No sequence available.

  • Chain 'Y':
    No sequence available.