PDB entry 2f53

View 2f53 on RCSB PDB site
Description: Directed Evolution of Human T-cell Receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without apparent cross-reactivity
Class: immune system
Keywords: T-cell Receptor, CDR2, Phage Display, Mutant, High Affinity, NY-ESO-1, IMMUNE SYSTEM
Deposited on 2005-11-25, released 2006-04-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.169
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: hla class I histocompatibility antigen
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f53a1, d2f53a2
  • Chain 'B':
    Compound: Beta-2-microglobulin
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P61769 (1-99)
      • cloning artifact (0)
      • engineered (91)
    Domains in SCOPe 2.08: d2f53b1, d2f53b2
  • Chain 'C':
    Compound: Cancer/testis antigen 1B
    Species: synthetic, synthetic
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: T-cell receptor, alpha chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2f53d1, d2f53d2
  • Chain 'E':
    Compound: T-cell receptor, beta chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 2F53 (0-242)
    Domains in SCOPe 2.08: d2f53e1, d2f53e2
  • Heterogens: NA, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2f53A (A:)
gshsmryfftsvsrpgrgeprfiavgyvddtqfvrfdsdaasqrmeprapwieqegpeyw
dgetrkvkahsqthrvdlgtlrgyynqseagshtvqrmygcdvgsdwrflrgyhqyaydg
kdyialkedlrswtaadmaaqttkhkweaahvaeqlraylegtcvewlrrylengketlq
rtdapkthmthhavsdheatlrcwalsfypaeitltwqrdgedqtqdtelvetrpagdgt
fqkwaavvvpsgqeqrytchvqheglpkpltlrwe

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2f53B (B:)
miqrtpkiqvysrhpaengksnflncyvsgfhpsdievdllkngeriekvehsdlsfskd
wsfyllyyteftptekdeyacrvnhvtlsqpcivkwdrdm

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2f53D (D:)
mkqevtqipaalsvpegenlvlncsftdsaiynlqwfrqdpgkgltslllipfwqreqts
grlnasldkssgrstlyiaasqpgdsatylcavrptsggsyiptfgrgtslivhpyiqnp
dpavyqlrdskssdksvclftdfdsqtnvsqskdsdvyitdkcvldmrsmdfksnsavaw
snksdfacanafn

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2f53E (E:)
nagvtqtpkfqvlktgqsmtlqcaqdmnheymswyrqdpgmglrlihysvsvgmtdqgev
pngynvsrsttedfplrllsaapsqtsvyfcassyvgntgelffgegsrltvledlknvf
ppevavfepseaeishtqkatlvclatgfypdhvelswwvngkevhsgvctdpqplkeqp
alndsryalssrlrvsatfwqdprnhfrcqvqfyglsendewtqdrakpvtqivsaeawg
rad