PDB entry 7b73

View 7b73 on RCSB PDB site
Description: Insight into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2.
Class: lyase
Keywords: thermostability, enantioselectivity changes, directed evolution, protein engineering, short-chain dehydrogenase/reductase enzyme superfamily, dehalogenase, lyase
Deposited on 2020-12-09, released 2021-04-07
The last revision prior to the SCOPe 2.07 freeze date was dated 2021-04-07, with a file datestamp of 2021-04-02.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Short-chain dehydrogenase/reductase SDR
    Species: gamma proteobacterium HTCC2207 [TaxId:314287]
    Gene: GB2207_06463
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1YQT8 (Start-242)
      • expression tag (0-15)
    Domains in SCOPe 2.07: d7b73a1, d7b73a2
  • Chain 'B':
    Compound: Short-chain dehydrogenase/reductase SDR
    Species: gamma proteobacterium HTCC2207 [TaxId:314287]
    Gene: GB2207_06463
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1YQT8 (Start-242)
      • expression tag (2-7)
    Domains in SCOPe 2.07: d7b73b1, d7b73b2
  • Chain 'C':
    Compound: Short-chain dehydrogenase/reductase SDR
    Species: gamma proteobacterium HTCC2207 [TaxId:314287]
    Gene: GB2207_06463
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1YQT8 (Start-242)
      • expression tag (4-7)
    Domains in SCOPe 2.07: d7b73c1, d7b73c2
  • Chain 'D':
    Compound: Short-chain dehydrogenase/reductase SDR
    Species: gamma proteobacterium HTCC2207 [TaxId:314287]
    Gene: GB2207_06463
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d7b73d_
  • Heterogens: GOL, NHE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7b73A (A:)
shhhhhhssglvprgshmnnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnllt
dpalpakiiqqaghidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpq
mierqggkilvmgsasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfv
dnptyfpeetkanpkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsgg
wav

    Sequence, based on observed residues (ATOM records): (download) 
    >7b73A (A:)
shhhhhhssglvprgsnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnlltdpa
lpakiiqqaghidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpqmie
rqggkilvmgsasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfvdnp
tyfpeetkanpkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsggwav

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7b73B (B:)
shhhhhhssglvprgshmnnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnllt
dpalpakiiqqaghidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpq
mierqggkilvmgsasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfv
dnptyfpeetkanpkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsgg
wav

    Sequence, based on observed residues (ATOM records): (download) 
    >7b73B (B:)
hhhhhsnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnlltdpalpakiiqqag
hidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpqmierqggkilvmg
sasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfvdnptyfpeetkan
pkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsggwav

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >7b73C (C:)
shhhhhhssglvprgshmnnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnllt
dpalpakiiqqaghidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpq
mierqggkilvmgsasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfv
dnptyfpeetkanpkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsgg
wav

    Sequence, based on observed residues (ATOM records): (download) 
    >7b73C (C:)
hhhsnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnlltdpalpakiiqqaghi
dvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpqmierqggkilvmgsa
salrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfvdnptyfpeetkanpk
fqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsggwav

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >7b73D (D:)
shhhhhhssglvprgshmnnsqlagkrilvtqadtfmgptlcevfaemgaeviadnnllt
dpalpakiiqqaghidvlvinlaipapftkgelvddsewsatfsavvdpmprlctavlpq
mierqggkilvmgsasalrgmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfv
dnptyfpeetkanpkfqerlkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsgg
wav

    Sequence, based on observed residues (ATOM records): (download) 
    >7b73D (D:)
nsqlagkrilvtqadtfmgptlcevfaemgaeviadnnlltdpalpakiiqqaghidvlv
inlaipapftkgelvddsewsatfsavvdpmprlctavlpqmierqggkilvmgsasalr
gmkrastysaargaqlsyvkamgvemapqgiqinaiaqnfvdnptyfpeetkanpkfqer
lkrdvplgrlvslredalfaaylcsdaadcfvgqvfpvsggwav