PDB entry 6ruf

View 6ruf on RCSB PDB site
Description: Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Glu
Class: hydrolase
Keywords: hydrolase
Deposited on 2019-05-28, released 2020-07-15
The last revision prior to the SCOPe 2.07 freeze date was dated 2020-07-15, with a file datestamp of 2020-07-10.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase
    Species: Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) [TaxId:273121]
    Gene: ansA, ansB, WS0660
    Database cross-references and differences (RAF-indexed):
    • Uniprot P50286 (0-327)
      • conflict (118)
    Domains in SCOPe 2.07: d6rufa_
  • Chain 'B':
    Compound: L-asparaginase
    Species: Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) [TaxId:273121]
    Gene: ansA, ansB, WS0660
    Database cross-references and differences (RAF-indexed):
    • Uniprot P50286 (0-327)
      • conflict (118)
    Domains in SCOPe 2.07: d6rufb_
  • Chain 'C':
    Compound: L-asparaginase
    Species: Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) [TaxId:273121]
    Gene: ansA, ansB, WS0660
    Database cross-references and differences (RAF-indexed):
    • Uniprot P50286 (0-327)
      • conflict (118)
    Domains in SCOPe 2.07: d6rufc_
  • Chain 'D':
    Compound: L-asparaginase
    Species: Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) [TaxId:273121]
    Gene: ansA, ansB, WS0660
    Database cross-references and differences (RAF-indexed):
    • Uniprot P50286 (0-327)
      • conflict (118)
    Domains in SCOPe 2.07: d6rufd_
  • Heterogens: GLU, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6rufA (A:)
kpqvtilatggtiagsgessvkssysagavtvdkllaavpaindlatikgeqissigsqe
mtgkvwlklakrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpg
ssmsadgpmnlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgk
igtvyygkveyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakg
iihagmgngnpfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvatesln
pqkarvllmlaltktsdreaiqkifsty

    Sequence, based on observed residues (ATOM records): (download) 
    >6rufA (A:)
kpqvtilatggtiagssagavtvdkllaavpaindlatikgeqissigsqemtgkvwlkl
akrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpgssmsadgpm
nlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgkigtvyygkv
eyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakgiihagmgng
npfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvateslnpqkarvllm
laltktsdreaiqkifsty

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6rufB (B:)
kpqvtilatggtiagsgessvkssysagavtvdkllaavpaindlatikgeqissigsqe
mtgkvwlklakrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpg
ssmsadgpmnlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgk
igtvyygkveyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakg
iihagmgngnpfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvatesln
pqkarvllmlaltktsdreaiqkifsty

    Sequence, based on observed residues (ATOM records): (download) 
    >6rufB (B:)
kpqvtilatggtiagssagavtvdkllaavpaindlatikgeqissigsqemtgkvwlkl
akrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpgssmsadgpm
nlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgkigtvyygkv
eyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakgiihagmgng
npfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvateslnpqkarvllm
laltktsdreaiqkifsty

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6rufC (C:)
kpqvtilatggtiagsgessvkssysagavtvdkllaavpaindlatikgeqissigsqe
mtgkvwlklakrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpg
ssmsadgpmnlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgk
igtvyygkveyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakg
iihagmgngnpfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvatesln
pqkarvllmlaltktsdreaiqkifsty

    Sequence, based on observed residues (ATOM records): (download) 
    >6rufC (C:)
kpqvtilatggtiasagavtvdkllaavpaindlatikgeqissigsqemtgkvwlklak
rvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpgssmsadgpmnl
ynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgkigtvyygkvey
ftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakgiihagmgngnp
fpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvateslnpqkarvllmla
ltktsdreaiqkifsty

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6rufD (D:)
kpqvtilatggtiagsgessvkssysagavtvdkllaavpaindlatikgeqissigsqe
mtgkvwlklakrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpg
ssmsadgpmnlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgk
igtvyygkveyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakg
iihagmgngnpfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvatesln
pqkarvllmlaltktsdreaiqkifsty

    Sequence, based on observed residues (ATOM records): (download) 
    >6rufD (D:)
kpqvtilatggtiagssagavtvdkllaavpaindlatikgeqissigsqemtgkvwlkl
akrvnellaqketeaviithgtdtmeetafflnltvksqkpvvlvgamrpgssmsadgpm
nlynavnvainkastnkgvvivmndeihaareatklnttavnafaspntgkigtvyygkv
eyftqsvrphtlasefdiskieelprvdilyahpddtdvlvnaalqagakgiihagmgng
npfpltqnalekaaksgvvvarssrvgsgsttqeaevddkklgfvateslnpqkarvllm
laltktsdreaiqkifsty