PDB entry 4tti

View 4tti on RCSB PDB site
Description: Crystal structure of double mutant E. Coli purine nucleoside phosphorylase with 4 FMC molecules
Class: transferase
Keywords: Purine nucleoside phosphorylase, formicyn A, transferase
Deposited on 2014-06-21, released 2015-07-08
The last revision prior to the SCOPe 2.07 freeze date was dated 2016-03-16, with a file datestamp of 2016-03-11.
Experiment type: XRAY
Resolution: 1.89 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
      • engineered mutation (216)
    Domains in SCOPe 2.07: d4ttia_
  • Chain 'B':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
      • engineered mutation (216)
    Domains in SCOPe 2.07: d4ttib_
  • Chain 'C':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
    Domains in SCOPe 2.07: d4ttic_
  • Chain 'D':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
      • engineered mutation (216)
    Domains in SCOPe 2.07: d4ttid_
  • Chain 'E':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
      • engineered mutation (216)
    Domains in SCOPe 2.07: d4ttie_
  • Chain 'F':
    Compound: Purine nucleoside phosphorylase deoD-type
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QST6 (0-236)
      • engineered mutation (203)
      • engineered mutation (216)
    Domains in SCOPe 2.07: d4ttif_
  • Heterogens: FMC, PO4, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4ttiA (A:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4ttiB (B:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4ttiC (C:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk

    Sequence, based on observed residues (ATOM records): (download) 
    >4ttiC (C:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahttfndmikialesvllgdk

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4ttiD (D:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4ttiE (E:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4ttiF (F:)
atphinaemgdfadvvlmpgdplrakyiaetfledarevnnvrgmlgftgtykgrkisvm
ghgmgipscsiytkelitdfgvkkiirvgscgavlphvklrdvvigmgactdskvnrirf
kdhdfaaiadfdmvrnavdaakalgidarvgnlfsadlfyspdgemfdvmekygilgvem
eaagiygvaaefgakaltictvsahirtheqttaaeaqttfndmikialesvllgdk