PDB entry 4qqy

View 4qqy on RCSB PDB site
Description: Crystal structure of T. fusca Cas3-ADP
Class: Hydrolase/DNA
Keywords: RISPR, Cas3, helicase, Hydrolase-DNA complex
Deposited on 2014-06-30, released 2014-08-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-09-24, with a file datestamp of 2014-09-19.
Experiment type: XRAY
Resolution: 3.12 Å
R-factor: 0.171
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated helicase, Cas3 family
    Species: Thermobifida fusca [TaxId:269800]
    Gene: Tfu_1593
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4qqya1, d4qqya2, d4qqya3, d4qqya4
  • Chain 'B':
    Compound: HD nuclease
  • Chain 'C':
    Compound: CRISPR-associated helicase, Cas3 family
    Species: Thermobifida fusca [TaxId:269800]
    Gene: Tfu_1593
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4qqyc1, d4qqyc2, d4qqyc3, d4qqyc4
  • Chain 'D':
    Compound: HD nuclease
  • Chain 'E':
    Compound: CRISPR-associated helicase, Cas3 family
    Species: Thermobifida fusca [TaxId:269800]
    Gene: Tfu_1593
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4qqye1, d4qqye2, d4qqye3, d4qqye4
  • Chain 'F':
    Compound: HD nuclease
  • Chain 'G':
    Compound: CRISPR-associated helicase, Cas3 family
    Species: Thermobifida fusca [TaxId:269800]
    Gene: Tfu_1593
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4qqyg1, d4qqyg2, d4qqyg3, d4qqyg4
  • Chain 'H':
    Compound: HD nuclease
  • Heterogens: FE, ADP

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4qqyA (A:)
mgsshhhhhhssglvprgshmpehdstddkhgippldlrfwakerglrgktyplvchsld
aaaaalvlwneylspglrdtiassmetdeehaghciafwaglhdigkltrefqqqiaidl
saypgeelsgeqrshaaatgkwlpfalpslgypngglvtglvaqmlgghhgtfhphpsfq
srnplaefgfssphwekqrhallhavfdatgrptppdmldgptasvvcglviladwlvsq
edfllerltslpadgsasalrahfetslrripslldaaglrpitvppatftesfphlskp
nglqaslakhlpclctgpglvlitapmgegkteaayhvadllgkatgrpgrflalptmat
adqmhtrlkeyaryrventdlprsstlallhsmawlnpdyapadlpgvskvlsnlghrdp
faatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvdeahavdpym
qvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsepqpvsevsyp
gwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvlakeltplvk
qggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrteitativdlfg
kegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcwrhehlgiin
rpqwakqpelvvltpeqngdadrapwfprswtsvyplallqrtytllrrrngapvqiped
vqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsfdv
dehvlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvart
ipvrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpck
glif

    Sequence, based on observed residues (ATOM records): (download) 
    >4qqyA (A:)
ppldlrfwakerglrgktyplvchsldaaaaalvlwneylspglrdtiassmetdeehag
hciafwaglhdigkltrefqqqiaidlsaypgeelsgeqrshaaatgkwlpfalpslgyp
ngglvtglvaqmlgghhgtfhphpsfqsrnplaefgfssphwekqrhallhavfdatgrp
tppdmldgptasvvcglviladwlvsqedfllerltslpadgsasalrahfetslrrips
lldaaglrpitvppatftesfphlskpnglqaslakhlpclctgpglvlitapmgegkte
aayhvadllgkatgrpgrflalptmatadqmhtrlkeyaryrveprsstlallhsmawln
pdyapaghrdpfaatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvv
vdeahavdpymqvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrs
epqpvsevsypgwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrst
vlakeltplvkqggcaaiicttvaeaqgvydllsqwfatapdlyllhsrfpnrqrteita
tivdlfgkegaqsgrrptavlvatqvveqsldldvdlmisdlapvslllqragrcwrheh
lgiinrpqwakqpelvvltpeqnrapwfprswtsvyplallqrtytllrrrngapvqipe
dvqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsfh
vlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtegrftmadcrdlvartipvrm
gpwasqltednhppeawresfylrdlvlipqrvtdvlptetggrewlldpckglif

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4qqyC (C:)
mgsshhhhhhssglvprgshmpehdstddkhgippldlrfwakerglrgktyplvchsld
aaaaalvlwneylspglrdtiassmetdeehaghciafwaglhdigkltrefqqqiaidl
saypgeelsgeqrshaaatgkwlpfalpslgypngglvtglvaqmlgghhgtfhphpsfq
srnplaefgfssphwekqrhallhavfdatgrptppdmldgptasvvcglviladwlvsq
edfllerltslpadgsasalrahfetslrripslldaaglrpitvppatftesfphlskp
nglqaslakhlpclctgpglvlitapmgegkteaayhvadllgkatgrpgrflalptmat
adqmhtrlkeyaryrventdlprsstlallhsmawlnpdyapadlpgvskvlsnlghrdp
faatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvdeahavdpym
qvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsepqpvsevsyp
gwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvlakeltplvk
qggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrteitativdlfg
kegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcwrhehlgiin
rpqwakqpelvvltpeqngdadrapwfprswtsvyplallqrtytllrrrngapvqiped
vqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsfdv
dehvlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvart
ipvrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpck
glif

    Sequence, based on observed residues (ATOM records): (download) 
    >4qqyC (C:)
ppldlrfwakerglrgktyplvchsldaaaaalvlwneylspglrdtiassmetdeehag
hciafwaglhdigkltrefqqqiaidlsaypgeelsgeqrshaaatgkwlpfalpslgyp
ngglvtglvaqmlgghhgtfhphpsfqsrnplaefgfssphwekqrhallhavfdatgrp
tppdmldgptasvvcglviladwlvsqedfllerltslpadgsasalrahfetslrrips
lldaaglrpitvppatftesfphlskpnglqaslakhlpclctgpglvlitapmgegkte
aayhvadllgkatgrpgrflalptmatadqmhtrlkeyaryrventrsstlallhsmawl
npdyapadpfaatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvd
eahavdpymqvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsep
qpvsevsypgwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvl
akeltplvkqggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrtei
tativdlfgkegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcw
rhehlgiinrpqwakqpelvvltpeqnapwfprswtsvyplallqrtytllrrrngapvq
ipedvqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltef
svlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvartip
vrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpckgl
if

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4qqyE (E:)
mgsshhhhhhssglvprgshmpehdstddkhgippldlrfwakerglrgktyplvchsld
aaaaalvlwneylspglrdtiassmetdeehaghciafwaglhdigkltrefqqqiaidl
saypgeelsgeqrshaaatgkwlpfalpslgypngglvtglvaqmlgghhgtfhphpsfq
srnplaefgfssphwekqrhallhavfdatgrptppdmldgptasvvcglviladwlvsq
edfllerltslpadgsasalrahfetslrripslldaaglrpitvppatftesfphlskp
nglqaslakhlpclctgpglvlitapmgegkteaayhvadllgkatgrpgrflalptmat
adqmhtrlkeyaryrventdlprsstlallhsmawlnpdyapadlpgvskvlsnlghrdp
faatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvdeahavdpym
qvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsepqpvsevsyp
gwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvlakeltplvk
qggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrteitativdlfg
kegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcwrhehlgiin
rpqwakqpelvvltpeqngdadrapwfprswtsvyplallqrtytllrrrngapvqiped
vqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsfdv
dehvlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvart
ipvrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpck
glif

    Sequence, based on observed residues (ATOM records): (download) 
    >4qqyE (E:)
ppldlrfwakerglrgktyplvchsldaaaaalvlwneylspglrdtiassmetdeehag
hciafwaglhdigkltrefqqqiaidlsaypgeelsgeqrshaaatgkwlpfalpslgyp
ngglvtglvaqmlgghhgtfhphpsfqsrnplaefgfssphwekqrhallhavfdatgrp
tppdmldgptasvvcglviladwlvsqedfllerltslpadgsasalrahfetslrrips
lldaaglrpitvppatftesfphlskpnglqaslakhlpclctgpglvlitapmgegkte
aayhvadllgkatgrpgrflalptmatadqmhtrlkeyaryrventrsstlallhsmawl
npdyapadpfaatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvd
eahavdpymqvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsep
qpvsevsypgwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvl
akeltplvkqggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrtei
tativdlfgkegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcw
rhehlgiinrpqwakqpelvvltpeqnrapwfprswtsvyplallqrtytllrrrngapv
qipedvqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlnglte
fvlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvartip
vrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpckgl
if

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >4qqyG (G:)
mgsshhhhhhssglvprgshmpehdstddkhgippldlrfwakerglrgktyplvchsld
aaaaalvlwneylspglrdtiassmetdeehaghciafwaglhdigkltrefqqqiaidl
saypgeelsgeqrshaaatgkwlpfalpslgypngglvtglvaqmlgghhgtfhphpsfq
srnplaefgfssphwekqrhallhavfdatgrptppdmldgptasvvcglviladwlvsq
edfllerltslpadgsasalrahfetslrripslldaaglrpitvppatftesfphlskp
nglqaslakhlpclctgpglvlitapmgegkteaayhvadllgkatgrpgrflalptmat
adqmhtrlkeyaryrventdlprsstlallhsmawlnpdyapadlpgvskvlsnlghrdp
faatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvdeahavdpym
qvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsepqpvsevsyp
gwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvlakeltplvk
qggcaaiicttvaeaqgvydllsqwfatlgedapdlyllhsrfpnrqrteitativdlfg
kegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcwrhehlgiin
rpqwakqpelvvltpeqngdadrapwfprswtsvyplallqrtytllrrrngapvqiped
vqqlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsfdv
dehvlatrfgagsvrvlcyyvdtagnrwldpectvefpeqgtgregrftmadcrdlvart
ipvrmgpwasqltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpck
glif

    Sequence, based on observed residues (ATOM records): (download) 
    >4qqyG (G:)
ppldlrfwakerglrgktyplvchsldaaaaalvlwneylspglrdtiassmetdeehag
hciafwaglhdigkltrefqqqiaidlsaypgeelsgeqrshaaatgkwlpfalpslgyp
ngglvtglvaqmlgghhgtfhphpsfqsrnplaefgfssphwekqrhallhavfdatgrp
tppdmldgptasvvcglviladwlvsqedfllerltslpadgsasalrahfetslrrips
lldaaglrpitvppatftesfphlskpnglqaslakhlpclctgpglvlitapmgegkte
aayhvadllgkatgrpgrflalptmatadqmhtrlkeyaryrvensstlallhsmawlnp
dyapapfaatdwlmgrkrgllapwavgtidqalmavlrakhnalrlfglagkvvvvdeah
avdpymqvlleqllrwlgtldvpvvllsatlhhsianslvkaylegargrrwnrsepqpv
sevsypgwlhvdarigkvtrssdvdplpiattprkplevrlvdvpvkegalnrstvlake
ltplvkqggcaaiicttvaeaqgvydllsqwfatapdlyllhsrfpnrqrteitativdl
fgkegaqsgrrptrgavlvatqvveqsldldvdlmisdlapvslllqragrcwrhehlgi
inrpqwakqpelvvltpeqnrapwfprswtsvyplallqrtytllrrrngapvqipedvq
qlvddvydddslaedleadmermgeelaqrglarnavipdpddaednlngltefsvlatr
fgagsvrvlcyyvagnrwldpectvefpeqgtgregrftmadcrdlvartipvrmgpwas
qltednhppeawresfylrdlvlipqrvtdegavlptetggrewlldpckglif

  • Chain 'H':
    No sequence available.