PDB entry 4gwe

View 4gwe on RCSB PDB site
Description: W116L-OYE1 complexed with (R)-carvone
Class: oxidoreductase
Keywords: reductase, enantioselectivity, flipped binding, carvone, enoate reductase, OYE, old yellow enzyme, biocatalysis, atomic resolution, TIM barrel, alpha/beta barrel, Oxidoreductase
Deposited on 2012-09-02, released 2012-10-03
The last revision prior to the SCOPe 2.03 freeze date was dated 2012-10-03, with a file datestamp of 2012-09-28.
Experiment type: XRAY
Resolution: 1.45 Å
R-factor: 0.118
AEROSPACI score: 0.72 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: nadph dehydrogenase 1
    Species: Saccharomyces pastorianus [TaxId:27292]
    Gene: OYE1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q02899
      • engineered mutation (116)
    Domains in SCOPe 2.03: d4gwea_
  • Heterogens: FMN, MG, 1PE, NA, CL, 07V, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4gweA (A:)
msfvkdfkpqalgdtnlfkpikignnellhravippltrmralhpgnipnrdwaveyytq
raqrpgtmiitegafispqaggydnapgvwseeqmvewtkifnaihekksfvwvqllvlg
waafpdnlardglrydsasdnvfmdaeqeakakkannpqhsltkdeikqyikeyvqaakn
siaagadgveihsangyllnqfldphsntrtdeyggsienrarftlevvdalveaighek
vglrlspygvfnsmsggaetgivaqyayvagelekrakagkrlafvhlveprvtnpflte
gegeyeggsndfvysiwkgpviragnfalhpevvreevkdkrtligygrffisnpdlvdr
lekglplnkydrdtfyqmsahgyidyptyeealklgwdkk

    Sequence, based on observed residues (ATOM records): (download) 
    >4gweA (A:)
sfvkdfkpqalgdtnlfkpikignnellhravippltrmralhpgnipnrdwaveyytqr
aqrpgtmiitegafispqaggydnapgvwseeqmvewtkifnaihekksfvwvqllvlgw
aafpdnlardglrydsasdnvfmdaeqeakakkannpqhsltkdeikqyikeyvqaakns
iaagadgveihsangyllnqfldphsntrtdeyggsienrarftlevvdalveaighekv
glrlspygvfnsmsggaetgivaqyayvagelekrakagkrlafvhlveprvtnpflteg
egeyeggsndfvysiwkgpviragnfalhpevvreevkdkrtligygrffisnpdlvdrl
ekglplnkydrdtfyqmsahgyidyptyeealklgwdk