PDB entry 3cqz

View 3cqz on RCSB PDB site
Description: Crystal structure of 10 subunit RNA polymerase II in complex with the inhibitor alpha-amanitin
Class: transcription/toxin
Keywords: transcription-toxin complex, alpha amanitin, toxin, inhibitor, polymerase, transferase, DNA binding, zinc-finger, phosphoprotein, transcription
Deposited on 2008-04-03, released 2008-07-22
The last revision prior to the SCOPe 2.03 freeze date was dated 2011-07-27, with a file datestamp of 2011-07-22.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.202
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase II subunit RPB1
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqza1
  • Chain 'B':
    Compound: DNA-directed RNA polymerase II subunit RPB2
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzb_
  • Chain 'C':
    Compound: DNA-directed RNA polymerase II subunit RPB3
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC1
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzf1
  • Chain 'H':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzh_
  • Chain 'I':
    Compound: DNA-directed RNA polymerase II subunit RPB9
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzj_
  • Chain 'K':
    Compound: DNA-directed RNA polymerase II subunit RPB11
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzk_
  • Chain 'L':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC4
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.03: d3cqzl1
  • Chain 'M':
    Compound: alpha-amanitin
    Species: AMANITA PHALLOIDES, synthetic [TaxId:67723]
    Database cross-references and differences (RAF-indexed):
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3cqzA (A:)
mvgqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgs
idrnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehne
lmrqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglk
lvgswkkdratgdadepelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclp
vppppvrpsisfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhv
atymdndiagqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnle
ldqvgvpksiaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskr
agdiqlqygwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspyna
dfdgdemnlhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtf
ieldqvlnmlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllsp
kdngmliidgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhn
gfstgigdtiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvr
flneardkagrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfv
drtlphfskddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrr
lvkaledimvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvd
llntdhtldpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvni
rriiqnaqqtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfcc
llrsrlatrrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmt
lntfhfagvaskkvtsgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaieh
ttlksvtiaseiyydpdprstvipedeeiiqlhfslldeeaeqsfdqqspwllrleldra
amndkdltmgqvgerikqtfkndlfviwsedndekliircrvvrpksldaeteaeedhml
kkientmlenitlrgveniervvmmkydrkvpsptgeyvkepewvletdgvnlsevmtvp
gidptriytnsfidimevlgieagraalykevynviasdgsyvnyrhmallvdvmttqgg
ltsvtrhgfnrsntgalmrcsfeetveilfeagasaelddcrgvsenvilgqmapigtga
fdvmideeslvkympeqkiteiedgqdggvtpysnesglvnadldvkdelmfsplvdsgs
ndamaggftayggadygeatspfgaygeaptspgfgvsspgfsptsptysptspayspts
psysptspsysptspsysptspsysptspsysptspsysptspsysptspsysptspsys
ptspsysptspsysptspsysptspsysptspsysptspaysptspsysptspsysptsp
sysptspsysptspnysptspsysptspgyspgspayspkqdeqkhnenensr

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzA (A:)
yssaplrtvkevqfglfspeevraisvakirfpetakigglndprlgsidrnlkcqtcqe
gmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehnelmrqalaikdsk
krfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglklvgswkkepelr
vlsteeilnifkhisvkdftslgfnevfsrpewmiltclpvppppvrptfkladilkani
sletlehngaphhaieeaesllqfhvatymdfsartvisgdpnleldqvgvpksiaktlt
ypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskragdiqlqygwkverh
imdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspynadfdgdemnlhvpqse
etraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtfieldqvlnmlywvpd
wdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllspkdngmliidgqiifg
vvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhngfstgigdtiadgpt
mreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvrflneardkagrlaev
nlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfvdrtlphfskddyspe
skgfvensylrgltpqefffhamggreglidtavktaetgyiqrrlvkaledimvhydnt
trnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvdllntdhtldpslles
gseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvnirriiqnaqqtfhidh
tkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfccllrsrlatrrvlqey
rltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmtlntfhfagvaskkvt
sgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaiehttlksvtiaseiyyd
pdprstvipedeeiiqlhfslldqqspwllrleldraamndkdltmgqvgerikqtfknd
lfviwsedndekliircrvveaeedhmlkkientmlenitlrgveniervvmmkydrkvp
sptgeyvkepewvletdgvnlsevmtvpgidptriytnsfidimevlgieagraalykev
ynviasdgsyvnyrhmallvdvmttqggltsvtrhgmrcsfeetveilfeagasaelddc
rgvsenvilgqmapigtgafdvmideesl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3cqzB (B:)
msdlansekyydedpygfedesapitaedswavisaffrekglvsqqldsfnqfvdytlq
diicedstlileqlaqhttesdnisrkyeisfgkiyvtkpmvnesdgvthalypqearlr
nltyssglfvdvkkrtyeaidvpgrelkyeliaeeseddsesgkvfigrlpimlrskncy
lseatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaei
rsalekgsrfistlqvklygregssartikatlpyikqdipiviifralgiipdgeileh
icydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkef
lphitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktl
fkkltkdifrymqrtveeahdfnmklainaktitsglkyalatgnwgeqkkamssragvs
qvlnrytysstlshlrrtntpigrdgklakprqlhnthwglvcpaetpegqacglvknls
lmscisvgtdpmpiitflsewgmepledyvphqspdatrvfvngvwhgvhrnparlmetl
rtlrrkgdinpevsmirdirekelkiftdagrvyrplfiveddeslghkelkvrkghiak
lmateyqdieggfedveeytwssllneglveyidaeeeesiliamqpedlepaeaneend
ldvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprntyqsamgkqamgv
fltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaiacysgynqedsmi
mnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtydkldddglia
pgvrvsgedviigkttpispdeeelgqrtayhskrdastplrstengivdqvlvttnqdg
lkfvkvrvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsr
mtvahliecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkk
lmaqiffgptyyqrlrhmvddkiharargpmqvltrqpvegrsrdgglrfgemerdcmia
hgaasflkerlmeasdafrvhicgicglmtviaklnhnqfeckgcdnkidiyqihipyaa
kllfqelmamnitprlytdrsrdf

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzB (B:)
desapitaedswavisaffrekglvsqqldsfnqfvdytlqdiicedstleisfgkiyvt
kpmvnesdgvthalypqearlrnltyssglfvdvkkesgkvfigrlpimlrskncylsea
tesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaeirsal
ekgistlqvklygregssartikatlpyikqdipiviifralgiipdgeilehicydvnd
wqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkeflphitql
egfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktlfkkltkd
ifrlainaktitsglkyalatgnwagvsqvlnrytysstlshlrrtntpigrdgklakpr
qlhnthwglvcpaetpegqacglvknlslmscisvgtdpmpiitflsewgmepledyvph
qspdatrvfvngvwhgvhrnparlmetlrtlrrkgdinpevsmirdirekelkiftdagr
vyrplfiveddeslghkelkvrkghiaklmateyqdveeytwssllneglveyidaeeee
siliamqpedlepdvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprnt
yqsamgkqamgvfltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaia
cysgynqedsmimnqssidrglfrslffrsymdqekkitetfekpqrtrmkhgtydkldd
dgliapgvrvsgedviigkttpskrdastplrstengivdqvlvttnqdglkfvkvrvrt
tkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsrmtvahliecl
lskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkklmaqiffgpt
yyqrlrhmvglrfgemerdcmiahgaasflkerlmeasdafrvhicgicglmtviahnqf
eckgcdnkidiyqihipyaakllfqelmamnitprlytdrs

  • Chain 'C':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3cqzF (F:)
msdyeeafndgnenfedfdvehfsdeetyeekpqfkdgettdangktivtggngpedfqq
heqirrktlkekaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplri
amkelaekkiplvirrylpdgsfedwsveelivdl

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzF (F:)
kaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplriamkelaekkip
lvirrylpdgsfedwsveelivdl

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >3cqzH (H:)
msntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtia
sslnledtpandssatrswrppqagdrsladdydyvmygtaykfeevskdliavyysfgg
llmrlegnyrnlnnlkqenayllirr

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzH (H:)
sntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtias
trswrppqagdrsladdydyvmygtaykfeevskvyysfggllmrleenayllirr

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >3cqzJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynplekrd

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynp

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >3cqzK (K:)
mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtlaaddaf

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzK (K:)
napdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaay
kvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtl

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >3cqzL (L:)
msregfqiptnldaaaagtsqartatlkyicaecssklslsrtdavrckdcghrillkar
tkrlvqfear

    Sequence, based on observed residues (ATOM records): (download) 
    >3cqzL (L:)
tlkyicaecssklslsrtdavrccghrillkartkrlvqfear

  • Chain 'M':
    No sequence available.