SCOPe 2.07: Structural Classification of Proteins

PDB entry 2xh4

View 2xh4 on RCSB PDB site
Description: engineering the enolase active site pocket: crystal structure of the s39a d321a mutant of yeast enolase 1
Class: lyase
Keywords: lyase, tim-barrel, enolase superfamily, glycolysis, gluconeogenesis, metal binding
Deposited on 2010-06-09, released 2010-08-25
The last revision prior to the SCOPe 2.07 freeze date was dated 2011-04-20, with a file datestamp of 2011-04-15.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: 0.236
AEROSPACI score: 0.5 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: enolase 1
Species: Saccharomyces cerevisiae [TaxId:4932]
Database cross-references and differences (RAF-indexed):
- Uniprot P00924 (0-435)
  - expression tag (436-437)
  - engineered mutation (38)
  - conflict (240)
  - engineered mutation (320)
Domains in SCOPe 2.07: d2xh4a1, d2xh4a2, d2xh4a3
Chain 'B':
Compound: enolase 1
Species: Saccharomyces cerevisiae [TaxId:4932]
Database cross-references and differences (RAF-indexed):
- Uniprot P00924 (0-435)
  - expression tag (436-437)
  - conflict (240)
  - engineered mutation (320)
Domains in SCOPe 2.07: d2xh4b1, d2xh4b2, d2xh4b3
Chain 'C':
Compound: enolase 1
Species: Saccharomyces cerevisiae [TaxId:4932]
Database cross-references and differences (RAF-indexed):
- Uniprot P00924 (0-435)
  - expression tag (436-437)
  - engineered mutation (38)
  - conflict (240)
  - engineered mutation (320)
Domains in SCOPe 2.07: d2xh4c1, d2xh4c2, d2xh4c3
Chain 'D':
Compound: enolase 1
Species: Saccharomyces cerevisiae [TaxId:4932]
Database cross-references and differences (RAF-indexed):
- Uniprot P00924 (0-435)
  - expression tag (436-437)
  - conflict (240)
  - engineered mutation (320)
Domains in SCOPe 2.07: d2xh4d1, d2xh4d2, d2xh4d3
Heterogens: MG, 2PG, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>2xh4A (A:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllhhhhhh

Sequence, based on observed residues (ATOM records): (download)

>2xh4A (A:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllh

Chain 'B':
Sequence, based on SEQRES records: (download)

>2xh4B (B:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllhhhhhh

Sequence, based on observed residues (ATOM records): (download)

>2xh4B (B:)
avskvyarsvydsrgnptvevelttekgvfrsivpsggvlhavknvndviapafvkanid
vkdqkavddflisldgtanksklganailgvslaasraaaaeknvplykhladlskskts
pyvlpvpflnvlnggshaggalalqefmiaptgaktfaealrigsevyhnlksltkkryg
asagnvgdeggvapniqtaeealdlivdaikaaghdgkikigldcasseffkdgkydldf
knnsdkskwltgpqladlyhslmkrypivsiedpfaeddweawshffktagiqivadalt
vtnpkriataiekkaadalllkvnqigtlsesikaaqdsfaagwgvmvshrsgetedtfi
adlvvglrtgqiktgaparserlaklnqllrieeelgdnavfagenfhhgdkllh

Chain 'C':
Sequence, based on SEQRES records: (download)

>2xh4C (C:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllhhhhhh

Sequence, based on observed residues (ATOM records): (download)

>2xh4C (C:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllh

Chain 'D':
Sequence, based on SEQRES records: (download)

>2xh4D (D:)
avskvyarsvydsrgnptvevelttekgvfrsivpsgaatgvhealemrdgdkskwmgkg
vlhavknvndviapafvkanidvkdqkavddflisldgtanksklganailgvslaasra
aaaeknvplykhladlsksktspyvlpvpflnvlnggshaggalalqefmiaptgaktfa
ealrigsevyhnlksltkkrygasagnvgdeggvapniqtaeealdlivdaikaaghdgk
ikigldcasseffkdgkydldfknpnsdkskwltgpqladlyhslmkrypivsiedpfae
ddweawshffktagiqivadaltvtnpkriataiekkaadalllkvnqigtlsesikaaq
dsfaagwgvmvshrsgetedtfiadlvvglrtgqiktgaparserlaklnqllrieeelg
dnavfagenfhhgdkllhhhhhh

Sequence, based on observed residues (ATOM records): (download)

>2xh4D (D:)
avskvyarsvydsrgnptvevelttekgvfrsivpsggvlhavknvndviapafvkanid
vkdqkavddflisldgtanksklganailgvslaasraaaaeknvplykhladlskskts
pyvlpvpflnvlnggshaggalalqefmiaptgaktfaealrigsevyhnlksltkkryg
asagnvgdeggvapniqtaeealdlivdaikaaghdgkikigldcasseffkdgkydldf
knnsdkskwltgpqladlyhslmkrypivsiedpfaeddweawshffktagiqivadalt
vtnpkriataiekkaadalllkvnqigtlsesikaaqdsfaagwgvmvshrsgetedtfi
adlvvglrtgqiktgaparserlaklnqllrieeelgdnavfagenfhhgdkllh