PDB entry 2is3

View 2is3 on RCSB PDB site
Description: Crystal Structure of Escherichia coli RNase T
Class: hydrolase
Keywords: RNase, ribonuclease, exoribonuclease, exonuclease, nuclease, hydrolase, stable RNA maturation, tRNA end-turnover
Deposited on 2006-10-16, released 2007-04-24
The last revision prior to the SCOPe 2.04 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 3.1 Å
R-factor: 0.197
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:83333]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • modified residue (45)
      • modified residue (52)
      • modified residue (116)
      • modified residue (128-129)
      • modified residue (172)
    Domains in SCOPe 2.04: d2is3a_
  • Chain 'B':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:83333]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • modified residue (45)
      • modified residue (52)
      • modified residue (116)
      • modified residue (128-129)
      • modified residue (172)
    Domains in SCOPe 2.04: d2is3b_
  • Chain 'C':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:83333]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • modified residue (45)
      • modified residue (52)
      • modified residue (116)
      • modified residue (128-129)
      • modified residue (172)
    Domains in SCOPe 2.04: d2is3c_
  • Chain 'D':
    Compound: Ribonuclease T
    Species: Escherichia coli [TaxId:83333]
    Gene: rnt
    Database cross-references and differences (RAF-indexed):
    • Uniprot P30014
      • modified residue (45)
      • modified residue (52)
      • modified residue (116)
      • modified residue (128-129)
      • modified residue (172)
    Domains in SCOPe 2.04: d2is3d_
  • Heterogens: SO4

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2is3A (A:)
msdnaqltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhf
hvepfvganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvah
nanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqa
hsalydtertavlfceivnrwkrlggwplsaaeev

    Sequence, based on observed residues (ATOM records): (download) 
    >2is3A (A:)
ltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhfhvepfv
ganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvahnanfdh
sfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqahsalyd
tertavlfceivnrwkrlggwpl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2is3B (B:)
msdnaqltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhf
hvepfvganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvah
nanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqa
hsalydtertavlfceivnrwkrlggwplsaaeev

    Sequence, based on observed residues (ATOM records): (download) 
    >2is3B (B:)
ltglcdrfrgfypvvidvetleiaaitlkmdeqgwlmpdttlhfhvepgavseyealhei
fkvvrkgikasgcnraimvahnanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglal
gqtvlskacqtagmdfdstqahsalydtertavlfceivnrwkrlggwplsa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2is3C (C:)
msdnaqltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhf
hvepfvganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvah
nanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqa
hsalydtertavlfceivnrwkrlggwplsaaeev

    Sequence, based on observed residues (ATOM records): (download) 
    >2is3C (C:)
ltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhfhvepfv
ganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvahnanfdh
sfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqahsalyd
tertavlfceivnrwkrlggwpl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2is3D (D:)
msdnaqltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhf
hvepfvganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvah
nanfdhsfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqa
hsalydtertavlfceivnrwkrlggwplsaaeev

    Sequence, based on observed residues (ATOM records): (download) 
    >2is3D (D:)
ltglcdrfrgfypvvidvetagfnaktdalleiaaitlkmdeqgwlmpdttlhfhvepfv
ganlqpealafngidpndpdrgavseyealheifkvvrkgikasgcnraimvahnanfdh
sfmmaaaeraslkrnpfhpfatfdtaalaglalgqtvlskacqtagmdfdstqahsalyd
tertavlfceivnrwkrlggwpl