PDB entry 2bga

View 2bga on RCSB PDB site
Description: bacillus cereus metallo-beta-lactamase (bcii) arg (121) cys mutant. solved at ph7 using 20 micromolar znso4 in the buffer. 1mm dtt was used as a reducing agent. cys221 is oxidized.
Class: hydrolase
Keywords: hydrolase, zinc, metallo-beta-lactamase, antibiotic resistance
Deposited on 2004-12-17, released 2005-03-31
The last revision prior to the SCOPe 2.04 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.1856
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.04: d2bgaa_
  • Chain 'B':
    Compound: beta-lactamase II
    Species: Bacillus cereus [TaxId:1396]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04190 (212-226)
      • engineered mutation (90)
    Domains in SCOPe 2.04: d2bgab_
  • Heterogens: AZI, GOL, ZN, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2bgaA (A:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bgaA (A:)
tviknetgtisisqlnknvwvhtelgsavpsnglvlntskglvlvdsswddkltkeliem
vekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdlqtv
tnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvnews
tsienvlkryrninavvpghgevgdkglllhtldllk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2bgaB (B:)
sqkvektviknetgtisisqlnknvwvhtelgsfngeavpsnglvlntskglvlvdsswd
dkltkeliemvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngy
eeplgdlqtvtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgn
vadayvnewstsienvlkryrninavvpghgevgdkglllhtldllk

    Sequence, based on observed residues (ATOM records): (download) 
    >2bgaB (B:)
ktviknetgtisisqlnknvwvhtelgsavpsnglvlntskglvlvdsswddkltkelie
mvekkfqkrvtdviithahadciggiktlkergikahstaltaelakkngyeeplgdlqt
vtnlkfgnmkvetfypgkghtednivvwlpqynilvggclvkstsakdlgnvadayvnew
stsienvlkryrninavvpghgevgdkglllhtldllk