PDB entry 1iun

View 1iun on RCSB PDB site
Description: meta-Cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) S103A mutant hexagonal
Class: hydrolase
Keywords: aromatic compounds, cumene, isopropylbenzene, meta-cleavage compound hydrolase, polychlorinated biphenyl degradation, Pseudomonas fluorescens IP01, alpha/beta-hydrolase, substrate specificity, cumene degradation, PCB, beta-ketolase
Deposited on 2002-03-06, released 2002-09-18
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: 0.196
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: meta-Cleavage product hydrolase
    Species: Pseudomonas fluorescens [TaxId:294]
    Gene: CUMD
    Database cross-references and differences (RAF-indexed):
    • Uniprot P96965
      • engineered (102)
    Domains in SCOPe 2.07: d1iuna_
  • Chain 'B':
    Compound: meta-Cleavage product hydrolase
    Species: Pseudomonas fluorescens [TaxId:294]
    Gene: CUMD
    Database cross-references and differences (RAF-indexed):
    • Uniprot P96965
      • engineered (102)
    Domains in SCOPe 2.07: d1iunb_
  • Heterogens: ACT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >1iunA (A:)
manleigksilaagvltnyhdvgegqpvilihgsgpgvsayanwrltipalskfyrviap
dmvgfgftdrpenynyskdswvdhiigimdaleiekahivgnafggglaiatalryserv
drmvlmgaagtrfdvteglnavwgytpsienmrnlldifaydrslvtdelarlryeasiq
pgfqesfssmfpeprqrwidalassdediktlpnetliihgredqvvplssslrlgelid
raqlhvfgrcghwtqieqtdrfnrlvveffneantpklvgrp

    Sequence, based on observed residues (ATOM records): (download) 
    >1iunA (A:)
anleigksilaagvltnyhdvgegqpvilihgsgpgvsayanwrltipalskfyrviapd
mvgfgftdrpenynyskdswvdhiigimdaleiekahivgnafggglaiatalryservd
rmvlmgaagtrfdvteglnavwgytpsienmrnlldifaydrslvtdelarlryeasiqp
gfqesfssmfpeprqrwidalassdediktlpnetliihgredqvvplssslrlgelidr
aqlhvfgrcghwtqieqtdrfnrlvveffnea

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >1iunB (B:)
manleigksilaagvltnyhdvgegqpvilihgsgpgvsayanwrltipalskfyrviap
dmvgfgftdrpenynyskdswvdhiigimdaleiekahivgnafggglaiatalryserv
drmvlmgaagtrfdvteglnavwgytpsienmrnlldifaydrslvtdelarlryeasiq
pgfqesfssmfpeprqrwidalassdediktlpnetliihgredqvvplssslrlgelid
raqlhvfgrcghwtqieqtdrfnrlvveffneantpklvgrp

    Sequence, based on observed residues (ATOM records): (download) 
    >1iunB (B:)
anleigksilaagvltnyhdvgegqpvilihgsgpgvsayanwrltipalskfyrviapd
mvgfgftdrpenynyskdswvdhiigimdaleiekahivgnafggglaiatalryservd
rmvlmgaagtrfdvteglnavwgytpsienmrnlldifaydrslvtdelarlryeasiqp
gfqesfssmfpeprqrwidalassdediktlpnetliihgredqvvplssslrlgelidr
aqlhvfgrcghwtqieqtdrfnrlvveffneantpk