PDB entry 7buk

View 7buk on RCSB PDB site
Description: T1 lipase mutant - 5M (D43E/T118N/E226D/E250L/N304E)
Class: hydrolase
Keywords: lipase, mutation, triglycerides, HYDROLASE
Deposited on 2020-04-07, released 2021-04-07
The last revision prior to the SCOPe 2.07 freeze date was dated 2021-04-07, with a file datestamp of 2021-04-02.
Experiment type: XRAY
Resolution: 2.64 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lipase
    Species: Geobacillus zalihae [TaxId:213419]
    Database cross-references and differences (RAF-indexed):
    • PDB 7BUK (Start-405)
    Domains in SCOPe 2.07: d7buka_
  • Chain 'B':
    Compound: Lipase
    Species: Geobacillus zalihae [TaxId:213419]
    Database cross-references and differences (RAF-indexed):
    • PDB 7BUK (Start-405)
    Domains in SCOPe 2.07: d7bukb_
  • Heterogens: ZN, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7bukA (A:)
mspisrhhhhhhlvprgsaslrandapivllhgftgwgreemfgfkywggvrgdieqwln
engyrtytlavgplssnwdraceayaqlvggtvdygaahaakhgharfgrtypgllpelk
rggrihiiahsqggqnarmlvsllengsqeereyakahnvslsplfegghhfvlsvttia
tphdgttlvnmvdftdrffdlqkavleaaavasnvpytsqvydfkldqwglrrqpgesfd
hyfdrlkrspvwtstdtarydlsvsgalklnqwvqaspntyylsfstertyrgaltgnhy
pelgmnafsavvcapflgsyreptlgiddrwlendgivntvsmngpkrgssdrivpydgt
lkkgvwndmgtynvdhleiigvdpnpsfdirafylrlaeqlaslrp

    Sequence, based on observed residues (ATOM records): (download) 
    >7bukA (A:)
slrandapivllhgftgwgreemfgfkywggvrgdieqwlnengyrtytlavgplssnwd
raceayaqlvggtvdygaahaakhgharfgrtypgllpelkrggrihiiahsqggqnarm
lvsllengsqeereyakahnvslsplfegghhfvlsvttiatphdgttlvnmvdftdrff
dlqkavleaaavasnvpytsqvydfkldqwglrrqpgesfdhyfdrlkrspvwtstdtar
ydlsvsgalklnqwvqaspntyylsfstertyrgaltgnhypelgmnafsavvcapflgs
yreptlgiddrwlendgivntvsmngpkrgssdrivpydgtlkkgvwndmgtynvdhlei
igvdpnpsfdirafylrlaeqlaslrp

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >7bukB (B:)
mspisrhhhhhhlvprgsaslrandapivllhgftgwgreemfgfkywggvrgdieqwln
engyrtytlavgplssnwdraceayaqlvggtvdygaahaakhgharfgrtypgllpelk
rggrihiiahsqggqnarmlvsllengsqeereyakahnvslsplfegghhfvlsvttia
tphdgttlvnmvdftdrffdlqkavleaaavasnvpytsqvydfkldqwglrrqpgesfd
hyfdrlkrspvwtstdtarydlsvsgalklnqwvqaspntyylsfstertyrgaltgnhy
pelgmnafsavvcapflgsyreptlgiddrwlendgivntvsmngpkrgssdrivpydgt
lkkgvwndmgtynvdhleiigvdpnpsfdirafylrlaeqlaslrp

    Sequence, based on observed residues (ATOM records): (download) 
    >7bukB (B:)
slrandapivllhgftgwgreemfgfkywggvrgdieqwlnengyrtytlavgplssnwd
raceayaqlvggtvdygaahaakhgharfgrtypgllpelkrggrihiiahsqggqnarm
lvsllengsqeereyakahnvslsplfegghhfvlsvttiatphdgttlvnmvdftdrff
dlqkavleaaavasnvpytsqvydfkldqwglrrqpgesfdhyfdrlkrspvwtstdtar
ydlsvsgalklnqwvqaspntyylsfstertyrgaltgnhypelgmnafsavvcapflgs
yreptlgiddrwlendgivntvsmngpkrgssdrivpydgtlkkgvwndmgtynvdhlei
igvdpnpsfdirafylrlaeqlaslrp