PDB entry 7bbd

View 7bbd on RCSB PDB site
Description: Crystal structure of monoubiquitinated TRIM21 RING (Ub-RING) In complex with ubiquitin charged Ube2N (Ube2N~Ub) and Ube2V2
Class: ligase
Keywords: E3 ubiquitin ligase, E2 conjugating enzyme, ubiquitin chain elongation, LIGASE
Deposited on 2020-12-17, released 2021-01-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-03-10, with a file datestamp of 2021-03-05.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Ubiquitin-conjugating enzyme E2 variant 2
    Species: Homo sapiens [TaxId:9606]
    Gene: UBE2V2, MMS2, UEV2
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q15819 (5-End)
      • expression tag (0-4)
    Domains in SCOPe 2.08: d7bbda1, d7bbda2
  • Chain 'B':
    Compound: Polyubiquitin-B,E3 ubiquitin-protein ligase TRIM21
    Species: Homo sapiens [TaxId:9606]
    Gene: TRIM21, RNF81, RO52, SSA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot J3QS39 (3-76)
      • expression tag (1-2)
      • linker (77-78)
    • Uniprot P19474 (79-End)
  • Chain 'C':
    Compound: Ubiquitin-conjugating enzyme E2 N
    Species: Homo sapiens [TaxId:9606]
    Gene: UBE2N, BLU
    Database cross-references and differences (RAF-indexed):
    • Uniprot P61088 (1-152)
      • expression tag (0)
      • engineered mutation (87)
      • engineered mutation (92)
    Domains in SCOPe 2.08: d7bbdc1, d7bbdc2
  • Chain 'D':
    Compound: Polyubiquitin-C
    Species: Homo sapiens [TaxId:9606]
    Gene: UBC
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d7bbdd_
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7bbdA (A:)
gsqefmavstgvkvprnfrlleeleegqkgvgdgtvswgleddedmtltrwtgmiigppr
tnyenriyslkvecgpkypeappsvrfvtkinmnginnssgmvdarsipvlakwqnsysi
kvvlqelrrlmmskenmklpqppegqtynn

    Sequence, based on observed residues (ATOM records): (download) 
    >7bbdA (A:)
gsqefmavstgvkvprnfrlleeleegqkgvgdgtvswgleddedmtltrwtgmiigppr
tnyenriyslkvecgpkypeappsvrfvtkinmnginnssgmvdarsipvlakwqnsysi
kvvlqelrrlmmskenmklpqppegqtyn

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >7bbdC (C:)
gmaglprriiketqrllaepvpgikaepdesnaryfhvviagpqdspfeggtfklelflp
eeypmaapkvrfmtkiyhpnvdklgrikldiladkwspalqirtvllsiqallsapnpdd
plandvaeqwktneaqaietarawtrlyamnni

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >7bbdD (D:)
mqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdyn
iqkestlhlvlrlrgg