PDB entry 6jst

View 6jst on RCSB PDB site
Description: Structure of Geobacillus kaustophilus lactonase, Y99P/D266N double mutant with bound 3-oxo-C8-HSL
Class: hydrolase
Keywords: Alpha-Beta Barrel, HYDROLASE
Deposited on 2019-04-08, released 2020-04-08
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-06-17, with a file datestamp of 2020-06-12.
Experiment type: XRAY
Resolution: 1.73 Å
R-factor: N/A
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus (strain HTA426) [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5
      • engineered mutation (102)
      • engineered mutation (269)
    Domains in SCOPe 2.08: d6jsta_
  • Chain 'B':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus (strain HTA426) [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5
      • engineered mutation (102)
      • engineered mutation (269)
    Domains in SCOPe 2.08: d6jstb_
  • Chain 'C':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus (strain HTA426) [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5
      • engineered mutation (102)
      • engineered mutation (269)
    Domains in SCOPe 2.08: d6jstc_
  • Chain 'D':
    Compound: phosphotriesterase
    Species: Geobacillus kaustophilus (strain HTA426) [TaxId:235909]
    Gene: GK1506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5KZU5
      • engineered mutation (102)
      • engineered mutation (269)
    Domains in SCOPe 2.08: d6jstd_
  • Heterogens: FE, ZN, OH, LAE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6jstA (A:)
gshnmaemvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaae
kmkrhgiqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllg
taeddiydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgavii
thtqegtmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgm
vgaptdeervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfv
niipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >6jstA (A:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfs

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6jstB (B:)
gshnmaemvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaae
kmkrhgiqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllg
taeddiydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgavii
thtqegtmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgm
vgaptdeervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfv
niipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >6jstB (B:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfs

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6jstC (C:)
gshnmaemvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaae
kmkrhgiqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllg
taeddiydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgavii
thtqegtmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgm
vgaptdeervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfv
niipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >6jstC (C:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfs

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6jstD (D:)
gshnmaemvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaae
kmkrhgiqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllg
taeddiydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgavii
thtqegtmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgm
vgaptdeervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfv
niipalknegirdevleqmfignpaalfsa

    Sequence, based on observed residues (ATOM records): (download) 
    >6jstD (D:)
emvetvcgpvpveqlgktlihehflfgypgfqgdvtrgtfredeslrvaveaaekmkrhg
iqtvvdptpndcgrnpaflrrvaeetglniicatgypyegegappyfqfrrllgtaeddi
ydmfmaeltegiadtgikagviklasskgriteyekmffraaaraqketgaviithtqeg
tmgpeqaayllehgadpkkivighmcgntdpdyhrktlaygvyiafdrfgiqgmvgaptd
eervrtllallrdgyekqimlshntvnvwlgrpftlpepfaemmknwhvehlfvniipal
knegirdevleqmfignpaalfs