PDB entry 5o3u

View 5o3u on RCSB PDB site
Description: Structural characterization of the fast and promiscuous macrocyclase from plant - PCY1-S562A bound to Presegetalin F1
Class: hydrolase
Keywords: segetalin biosynthesis, prolyl oligopeptidase, macrocyclase, peptidase, beta-propeller, closed form, hydrolase
Deposited on 2017-05-25, released 2018-02-07
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-03-28, with a file datestamp of 2018-03-23.
Experiment type: XRAY
Resolution: 1.86 Å
R-factor: N/A
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Peptide cyclase 1
    Species: Vaccaria hispanica [TaxId:39387]
    Gene: Pcy1
    Database cross-references and differences (RAF-indexed):
    • Uniprot R4P353 (0-723)
      • engineered mutation (561)
    Domains in SCOPe 2.07: d5o3ua1, d5o3ua2
  • Chain 'B':
    Compound: Peptide cyclase 1
    Species: Vaccaria hispanica [TaxId:39387]
    Gene: Pcy1
    Database cross-references and differences (RAF-indexed):
    • Uniprot R4P353 (Start-723)
      • engineered mutation (561)
    Domains in SCOPe 2.07: d5o3ub1, d5o3ub2
  • Chain 'C':
    Compound: Peptide cyclase 1
    Species: Vaccaria hispanica [TaxId:39387]
    Gene: Pcy1
    Database cross-references and differences (RAF-indexed):
    • Uniprot R4P353 (Start-723)
      • engineered mutation (561)
    Domains in SCOPe 2.07: d5o3uc1, d5o3uc2
  • Chain 'D':
    Compound: Peptide cyclase 1
    Species: Vaccaria hispanica [TaxId:39387]
    Gene: Pcy1
    Database cross-references and differences (RAF-indexed):
    • Uniprot R4P353 (Start-723)
      • engineered mutation (561)
    Domains in SCOPe 2.07: d5o3ud1, d5o3ud2
  • Chain 'L':
    Compound: Putative presegetalin F1
    Species: Vaccaria hispanica [TaxId:39387]
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: Putative presegetalin F1
    Species: Vaccaria hispanica [TaxId:39387]
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Putative presegetalin F1
    Species: Vaccaria hispanica [TaxId:39387]
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: Putative presegetalin F1
    Species: Vaccaria hispanica [TaxId:39387]
    Database cross-references and differences (RAF-indexed):
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5o3uA (A:)
matsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvl
eecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevly
dpnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfsp
aiwthdnkgffycpypplkegedhmtrsavnqearyhflgtdqsedillwrdlenpahhl
kcqitddgkyfllyildgcddankvycldltklpnglesfrgredsapfmklidsfdasy
taiandgsvftfqtnkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilr
ylsdvkhvleirdlesgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdl
kndptqlkifresvvpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhg
yggfginmmptfsasrivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfis
aaeylissgytkarrvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlg
ylwtgdygcsdkeeefkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplh
sfkllatmqhvlctsledspqknpiiariqrkaahygratmtqiaevadrygfmakalea
pwid

    Sequence, based on observed residues (ATOM records): (download) 
    >5o3uA (A:)
matsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvl
eecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevly
dpnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfsp
aiwthdnkgffycpypplsavnqearyhflgtdqsedillwrdlenpahhlkcqitddgk
yfllyildgcddankvycldltklpnglesfrsapfmklidsfdasytaiandgsvftfq
tnkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilrylsdvkhvleird
lesgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdlkndptqlkifres
vvpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhgyggfginmmptfs
asrivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfisaaeylissgytka
rrvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlgylwtgdygcsdke
eefkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplhsfkllatmqhvlc
tsledspqknpiiariqrkaahygratmtqiaevadrygfmakaleapwid

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5o3uB (B:)
matsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvl
eecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevly
dpnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfsp
aiwthdnkgffycpypplkegedhmtrsavnqearyhflgtdqsedillwrdlenpahhl
kcqitddgkyfllyildgcddankvycldltklpnglesfrgredsapfmklidsfdasy
taiandgsvftfqtnkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilr
ylsdvkhvleirdlesgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdl
kndptqlkifresvvpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhg
yggfginmmptfsasrivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfis
aaeylissgytkarrvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlg
ylwtgdygcsdkeeefkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplh
sfkllatmqhvlctsledspqknpiiariqrkaahygratmtqiaevadrygfmakalea
pwid

    Sequence, based on observed residues (ATOM records): (download) 
    >5o3uB (B:)
gfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvleece
lidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevlydpnl
reggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfspaiwt
hdnkgffycpyppavnqearyhflgtdqsedillwrdlenpahhlkcqitddgkyfllyi
ldgcddankvycldltklpnglesfsapfmklidsfdasytaiandgsvftfqtnkdapr
kklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilrylsdvkhvleirdlesgalq
hrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdlndptqlkifresvvpdfdrs
efevkqvfvpskdgtkipifiaarkgisldgshpcemhgyggfginmmptfsasrivflk
hlggvfclanirgggeygeewhkagfrdkkqnvfddfisaaeylissgytkarrvaiegg
anggllvaacinqrpdlfgcaeancgvmdmlrfhkftlgylwtgdygcsdkeeefkwlik
yspihnvrrpweqpgneetqypatmiltadhddrvvplhsfkllatmqhvlctsledspq
knpiiariqrkaahygratmtqiaevadrygfmakaleapwid

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5o3uC (C:)
matsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvl
eecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevly
dpnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfsp
aiwthdnkgffycpypplkegedhmtrsavnqearyhflgtdqsedillwrdlenpahhl
kcqitddgkyfllyildgcddankvycldltklpnglesfrgredsapfmklidsfdasy
taiandgsvftfqtnkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilr
ylsdvkhvleirdlesgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdl
kndptqlkifresvvpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhg
yggfginmmptfsasrivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfis
aaeylissgytkarrvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlg
ylwtgdygcsdkeeefkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplh
sfkllatmqhvlctsledspqknpiiariqrkaahygratmtqiaevadrygfmakalea
pwid

    Sequence, based on observed residues (ATOM records): (download) 
    >5o3uC (C:)
gfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvleece
lidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevlydpnl
reggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfspaiwt
hdnkgffycpyppavnqearyhflgtdqsedillwrdlenpahhlkcqitddgkyfllyi
ldgcddankvycldltklpnglesfrdsapfmklidsfdasytaiandgsvftfqtnkda
prkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilrylsdvkhvleirdlesga
lqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdlkndptqlkifresvvpdf
drsefevkqvfvpskdgtkipifiaarkgisldgshpcemhgyggfginmmptfsasriv
flkhlggvfclanirgggeygeewhkagfrdkkqnvfddfisaaeylissgytkarrvai
egganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlgylwtgdygcsdkeeefkw
likyspihnvrrpweqpgneetqypatmiltadhddrvvplhsfkllatmqhvlctsled
spqknpiiariqrkaahygratmtqiaevadrygfmakaleapwid

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5o3uD (D:)
matsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvl
eecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevly
dpnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfsp
aiwthdnkgffycpypplkegedhmtrsavnqearyhflgtdqsedillwrdlenpahhl
kcqitddgkyfllyildgcddankvycldltklpnglesfrgredsapfmklidsfdasy
taiandgsvftfqtnkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilr
ylsdvkhvleirdlesgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdl
kndptqlkifresvvpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhg
yggfginmmptfsasrivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfis
aaeylissgytkarrvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlg
ylwtgdygcsdkeeefkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplh
sfkllatmqhvlctsledspqknpiiariqrkaahygratmtqiaevadrygfmakalea
pwid

    Sequence, based on observed residues (ATOM records): (download) 
    >5o3uD (D:)
atsgfskplhyppvrrdetvvddyfgvkvadpyrwledpnseetkefvdnqeklansvle
ecelidkfkqkiidfvnfprcgvpfrrankyfhfynsglqaqnvfqmqddldgkpevlyd
pnlreggrsglslysvsedakyfafgihsgltewvtikilktedrsylpdtlewvkfspa
iwthdnkgffycpyppavnqearyhflgtdqsedillwrdlenpahhlkcqitddgkyfl
lyildgcddankvycldltklpnglesfrgdsapfmklidsfdasytaiandgsvftfqt
nkdaprkklvrvdlnnpsvwtdlvpeskkdllesahavnenqlilrylsdvkhvleirdl
esgalqhrlpidigsvdgitarrrdsvvffkftsiltpgivyqcdlkndptqlkifresv
vpdfdrsefevkqvfvpskdgtkipifiaarkgisldgshpcemhgyggfginmmptfsa
srivflkhlggvfclanirgggeygeewhkagfrdkkqnvfddfisaaeylissgytkar
rvaiegganggllvaacinqrpdlfgcaeancgvmdmlrfhkftlgylwtgdygcsdkee
efkwlikyspihnvrrpweqpgneetqypatmiltadhddrvvplhsfkllatmqhvlct
sledspqknpiiariqrkaahygratmtqiaevadrygfmakaleapwid

  • Chain 'L':
    No sequence available.

  • Chain 'M':
    No sequence available.

  • Chain 'N':
    No sequence available.

  • Chain 'O':
    No sequence available.