PDB entry 5aou

View 5aou on RCSB PDB site
Description: Structure of the engineered retro-aldolase RA95.5-8F apo
Class: lyase
Keywords: lyase, retro-aldolase, protein engineering, enzyme design, directed evolution
Deposited on 2015-09-11, released 2016-08-17
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-03-01, with a file datestamp of 2017-02-24.
Experiment type: XRAY
Resolution: 1.1 Å
R-factor: N/A
AEROSPACI score: 0.73 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: indole-3-glycerol phosphate synthase
    Species: Sulfolobus solfataricus [TaxId:2287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q06121 (Start-244)
      • expression tag (245-248)
      • engineered mutation (9)
      • engineered mutation (21-22)
      • engineered mutation (50)
      • engineered mutation (52)
      • engineered mutation (69)
      • engineered mutation (71)
      • engineered mutation (74)
      • engineered mutation (82)
      • engineered mutation (89)
      • engineered mutation (94)
      • engineered mutation (109)
      • engineered mutation (134)
      • engineered mutation (150)
      • engineered mutation (158)
      • engineered mutation (177)
      • engineered mutation (179)
      • engineered mutation (181-183)
      • engineered mutation (186)
      • engineered mutation (208-213)
      • engineered mutation (215)
      • engineered mutation (230)
      • engineered mutation (232)
    Domains in SCOPe 2.07: d5aoua1, d5aoua2
  • Heterogens: EDO, PO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5aouA (A:)
mprylkgwledvvqlslrrpsvhasrqrpiislnerilefnkrnitaiiayylrkspsgl
dverdpieyakymepyavglsikteekyfdgsyemlrkiassvsipilmndfivkesqid
daynlgadtvlliveiltereleslleyargygmeplilindendldialrigarfitiy
smnfetgeinkenqrklismipsnvvkvplldffepneieelrklgvnafmissslmrnp
ekikeliegslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5aouA (A:)
prylkgwledvvqlslrrpsvhasrqrpiislnerilefnkrnitaiiayylrksprdpi
eyakymepyavglsikteekyfdgsyemlrkiassvsipilmndfivkesqiddaynlga
dtvlliveiltereleslleyargygmeplilindendldialrigarfitiysmnfetg
einkenqrklismipsnvvkvplldffepneieelrklgvnafmissslmrnpekikeli
eg