PDB entry 4x81

View 4x81 on RCSB PDB site
Description: MycF mycinamicin III 3'-O-methyltransferase (E35Q, M56A, E139A variant) in complex with Mg, SAH and mycinamicin VI (MycE substrate)
Class: Transferase/Antibiotic
Keywords: macrolide, methyltransferase, antibiotic, natural product, Transferase-Antibiotic complex
Deposited on 2014-12-09, released 2015-03-04
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-05-27, with a file datestamp of 2015-05-22.
Experiment type: XRAY
Resolution: 1.59 Å
R-factor: N/A
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Mycinamicin III 3''-O-methyltransferase
    Species: Micromonospora griseorubida [TaxId:28040]
    Gene: mycF
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q49492
      • engineered mutation (54)
      • engineered mutation (75)
      • engineered mutation (158)
    Domains in SCOPe 2.06: d4x81a_
  • Chain 'B':
    Compound: Mycinamicin III 3''-O-methyltransferase
    Species: Micromonospora griseorubida [TaxId:28040]
    Gene: mycF
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q49492
      • engineered mutation (54)
      • engineered mutation (75)
      • engineered mutation (158)
    Domains in SCOPe 2.06: d4x81b_
  • Heterogens: SAH, DMS, MG, MVI, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4x81A (A:)
mgsshhhhhhssglvprgshmspstgvelyldllkrtvsnfiyqdathvaglitqaafve
earesgedyptvahtaigmkrlnnlqhcvesalrdgvpgdvletgvwrggacifargilk
aydvrdrtvwvadsfqgfpkitdddhpmdaemnlhqynaavdlptslatvqrnfsrygll
ddqvrflpgwfkdtmptapferlavlrmdgdsygatmdvlthayprlspggfaiiddyci
pacreavheyrdrhgisdeiveidrqgvywrrsa

    Sequence, based on observed residues (ATOM records): (download) 
    >4x81A (A:)
stgvelyldllkrtvsnfiyqdathvaglitqaafveearesgedyptvahtaigmkrln
nlqhcvesalrdgvpgdvletgvwrggacifargilkaydvrdrtvwvadsfqgfpkitd
ddhpmdaemnlhqynaavdlptslatvqrnfsrygllddqvrflpgwfkdtmptapferl
avlrmdgdsygatmdvlthayprlspggfaiiddycipacreavheyrdrhgisdeivei
drqgvywrrs

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4x81B (B:)
mgsshhhhhhssglvprgshmspstgvelyldllkrtvsnfiyqdathvaglitqaafve
earesgedyptvahtaigmkrlnnlqhcvesalrdgvpgdvletgvwrggacifargilk
aydvrdrtvwvadsfqgfpkitdddhpmdaemnlhqynaavdlptslatvqrnfsrygll
ddqvrflpgwfkdtmptapferlavlrmdgdsygatmdvlthayprlspggfaiiddyci
pacreavheyrdrhgisdeiveidrqgvywrrsa

    Sequence, based on observed residues (ATOM records): (download) 
    >4x81B (B:)
stgvelyldllkrtvsnfiyqdathvaglitqaafveearesgedyptvahtaigmkrln
nlqhcvesalrdgvpgdvletgvwrggacifargilkaydvrdrtvwvadsfqgfpkitd
ddhpmdaemnlhqynaavdlptslatvqrnfsrygllddqvrflpgwfkdtmptapferl
avlrmdgdsygatmdvlthayprlspggfaiiddycipacreavheyrdrhgisdeivei
drqgvywrrs