PDB entry 4wuo

View 4wuo on RCSB PDB site
Description: Structure of the E270A Mutant Isopropylmalate dehydrogenase from Thermus thermophilus in complex with IPM, Mn and NADH
Class: oxidoreductase
Keywords: isopropylmalate dehydrogenase, ipmdh, oxidoreductase, mutant
Deposited on 2014-11-03, released 2014-11-12
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-02-11, with a file datestamp of 2015-02-06.
Experiment type: XRAY
Resolution: 2.05 Å
R-factor: 0.173
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 3-isopropylmalate dehydrogenase
    Species: Thermus thermophilus [TaxId:300852]
    Gene: leuB, TTHA1230
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5SIY4 (3-347)
      • expression tag (1-2)
      • engineered mutation (272)
      • expression tag (348-349)
    Domains in SCOPe 2.06: d4wuoa1, d4wuoa2, d4wuoa3
  • Chain 'B':
    Compound: 3-isopropylmalate dehydrogenase
    Species: Thermus thermophilus [TaxId:300852]
    Gene: leuB, TTHA1230
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5SIY4 (3-347)
      • engineered mutation (272)
    Domains in SCOPe 2.06: d4wuob_
  • Heterogens: IPM, NAD, MN, K, EOH, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4wuoA (A:)
masmkvavlpgdgigpevteaalkvlraldeaeglglayevfpfggaaidafgepfpept
rkgveeaeavllgsvggpkwdglprkirpetgllslrksqdlfanlrpakvfpglerlsp
lkeeiargvdvlivreltggiyfgeprgmseaeawnteryskpevervarvafeaarkrr
khvvsvdkanvlevgefwrktveevgrgypdvalehqyvdamamhlvrsparfdvvvtgn
ifgdilsdlasvlpgslgllpsaslgrgtpvfapvhgsapdiagkgianptaailsaamm
lehafglvelarkvedavakalletpppdlggsagteaftatvlrhlaaaalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4wuoA (A:)
asmkvavlpgdgigpevteaalkvlraldeaeglglayevfpfggaaidafgepfpeptr
kgveeaeavllgsvggpkwdglprkirpetgllslrksqdlfanlrpakvfpglerlspl
keeiargvdvlivreltggiyfgeprgmseaeawnteryskpevervarvafeaarkrrk
hvvsvdkanvlevgefwrktveevgrgypdvalehqyvdamamhlvrsparfdvvvtgni
fgdilsdlasvlpgslgllpsaslgrgtpvfapvhgsapdiagkgianptaailsaamml
ehafglvelarkvedavakalletpppdlggsagteaftatvlrhlaaa

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4wuoB (B:)
masmkvavlpgdgigpevteaalkvlraldeaeglglayevfpfggaaidafgepfpept
rkgveeaeavllgsvggpkwdglprkirpetgllslrksqdlfanlrpakvfpglerlsp
lkeeiargvdvlivreltggiyfgeprgmseaeawnteryskpevervarvafeaarkrr
khvvsvdkanvlevgefwrktveevgrgypdvalehqyvdamamhlvrsparfdvvvtgn
ifgdilsdlasvlpgslgllpsaslgrgtpvfapvhgsapdiagkgianptaailsaamm
lehafglvelarkvedavakalletpppdlggsagteaftatvlrhlaaaalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4wuoB (B:)
mkvavlpgdgigpevteaalkvlraldeaeglglayevfpfggaaidafgepfpeptrkg
veeaeavllgsvggpkwdglprkirpetgllslrksqdlfanlrpakvfpglerlsplke
eiargvdvlivreltggiyfgeprgmseaeawnteryskpevervarvafeaarkrrkhv
vsvdkanvlevgefwrktveevgrgypdvalehqyvdamamhlvrsparfdvvvtgnifg
dilsdlasvlpgslgllpsaslgrgtpvfapvhgsapdiagkgianptaailsaammleh
afglvelarkvedavakalletpppdlggsagteaftatvlrhla