PDB entry 4uui

View 4uui on RCSB PDB site
Description: A case study for twinned data analysis: multiple crystal forms of the enzyme N-acetyl-neuraminic lyase
Class: lyase
Keywords: lyase, twinning, directed evolution, n-acetylneuraminic acid lyase, substrate specificity, protein engineering, aldolase
Deposited on 2014-07-29, released 2015-08-12
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-08-12, with a file datestamp of 2015-08-07.
Experiment type: XRAY
Resolution: 1.79 Å
R-factor: N/A
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (4-7)
      • engineered mutation (143)
      • engineered mutation (198)
    Domains in SCOPe 2.06: d4uuia1, d4uuia2
  • Chain 'B':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • expression tag (5-7)
      • engineered mutation (143)
      • engineered mutation (198)
    Domains in SCOPe 2.06: d4uuib1, d4uuib2
  • Chain 'C':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (5-7)
      • engineered mutation (143)
      • engineered mutation (198)
    Domains in SCOPe 2.06: d4uuic1, d4uuic2
  • Chain 'D':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (5-7)
      • engineered mutation (143)
      • engineered mutation (198)
    Domains in SCOPe 2.06: d4uuid1, d4uuid2
  • Heterogens: PYR, NA, 1PE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4uuiA (A:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >4uuiA (A:)
hhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqsls
ereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfee
hcdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqi
rrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqt
ecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4uuiB (B:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >4uuiB (B:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4uuiC (C:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >4uuiC (C:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4uuiD (D:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >4uuiD (D:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvfnipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg