PDB entry 4m81

View 4m81 on RCSB PDB site
Description: The structure of E292S glycosynthase variant of exo-1,3-beta-glucanase from Candida albicans complexed with 1-fluoro-alpha-D-glucopyranoside (donor) and p-nitrophenyl beta-D-glucopyranoside (acceptor) at 1.86A resolution
Class: hydrolase
Keywords: tim barrel, glycoside hydrolase family 5, glycoside hydrolase, cell wall hydrolase, glycosynthase, hydrolase, protein-carbohydrate interaction
Deposited on 2013-08-12, released 2014-06-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 1.86 Å
R-factor: N/A
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: exo-1,3-beta-glucanase
    Species: Candida albicans [TaxId:237561]
    Gene: CaO19.10507, XOG, XOG1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5AI63 (Start-398)
      • see remark 999 (62)
      • engineered mutation (290)
      • see remark 999 (327)
    Domains in SCOPe 2.08: d4m81a_
  • Heterogens: GLF, PNW, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4m81A (A:)
gghnvawdydnnvirgvnlggwfvlepymtpslfepfqngndqsgvpvdeyhwtqtlgke
aalrilqkhwstwiteqdfkqisnlglnfvripigywafqlldndpyvqgqvqylekalg
warknnirvwidlhgapgsqngfdnsglrdsynfqngdntqvtlnvlntifkkyggneys
dvvigiellneplgpvlnmdklkqffldgynslrqtgsvtpviihdafqvfgywnnfltv
aegqwnvvvdhhhyqvfsggelsrnindhisvacnwgwdakkeshwnvagswsaaltdca
kwlngvnrgaryegaydnapyigscqplldisqwsdehktdtrryieaqldafeytggwv
fwswktenapewsfqtltynglfpqpvtdrqfpnqcgfh

    Sequence, based on observed residues (ATOM records): (download) 
    >4m81A (A:)
awdydnnvirgvnlggwfvlepymtpslfepfqngndqsgvpvdeyhwtqtlgkeaalri
lqkhwstwiteqdfkqisnlglnfvripigywafqlldndpyvqgqvqylekalgwarkn
nirvwidlhgapgsqngfdnsglrdsynfqngdntqvtlnvlntifkkyggneysdvvig
iellneplgpvlnmdklkqffldgynslrqtgsvtpviihdafqvfgywnnfltvaegqw
nvvvdhhhyqvfsggelsrnindhisvacnwgwdakkeshwnvagswsaaltdcakwlng
vnrgaryegaydnapyigscqplldisqwsdehktdtrryieaqldafeytggwvfwswk
tenapewsfqtltynglfpqpvtdrqfpnqcgfh