PDB entry 4lnu

View 4lnu on RCSB PDB site
Description: Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin
Class: cell cycle/motor protein
Keywords: ALPHA-TUBULIN, APO-KINESIN, BETA-TUBULIN, DARPIN, KINESIN, MICROTUBULE, TUBULIN, CELL CYCLE-MOTOR PROTEIN complex
Deposited on 2013-07-12, released 2014-12-03
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-12-03, with a file datestamp of 2014-11-28.
Experiment type: XRAY
Resolution: 2.19 Å
R-factor: 0.157
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tubulin alpha chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • PDB 4LNU (0-End)
    Domains in SCOPe 2.07: d4lnua1, d4lnua2
  • Chain 'B':
    Compound: Tubulin beta chain
    Species: Ovis aries [TaxId:9940]
    Database cross-references and differences (RAF-indexed):
    • PDB 4LNU (0-End)
    Domains in SCOPe 2.07: d4lnub1, d4lnub2
  • Chain 'D':
    Compound: Designed ankyrin repeat protein (DARPIN) D1
    Species: artificial gene [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 4LNU (Start-168)
    Domains in SCOPe 2.07: d4lnud_
  • Chain 'K':
    Compound: kinesin-1 heavy chain
    Species: Homo sapiens [TaxId:9606]
    Gene: KIF5B
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33176
      • engineered mutation (64)
      • engineered mutation (167)
      • engineered mutation (173)
      • engineered mutation (293)
  • Heterogens: GTP, MG, SO4, GDP, MES, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4lnuA (A:)
    mrecisihvgqagvqignacwelyclehgiqpdgqmpsdktigggddsfntffsetgagk
    hvpravfvdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvld
    rirkladqctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvsta
    vvepynsiltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssita
    slrfdgalnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepan
    qmvkcdprhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpp
    tvvpggdlakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefse
    aredmaalekdyeevgvdsvegegeeegeey
    

    Sequence, based on observed residues (ATOM records): (download)
    >4lnuA (A:)
    mrecisihvgqagvqignacwelyclehgiqpdgqmpsdktgddsfntffsetgagkhvp
    ravfvdleptvidevrtgtyrqlfhpeqlitgkedaannyarghytigkeiidlvldrir
    kladqctglqgflvfhsfgggtgsgftsllmerlsvdygkksklefsiypapqvstavve
    pynsiltthttlehsdcafmvdneaiydicrrnldierptytnlnrlisqivssitaslr
    fdgalnvdltefqtnlvpyprihfplatyapvisaekayheqlsvaeitnacfepanqmv
    kcdprhgkymaccllyrgdvvpkdvnaaiatiktkrsiqfvdwcptgfkvginyqpptvv
    pggdlakvqravcmlsnttaiaeawarldhkfdlmyakrafvhwyvgegmeegefseare
    dmaalekdyeevgv
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >4lnuB (B:)
    mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
    prailvdlepgtmdsvrsgpfgqifrpdnfifgqsgagnnwakghytegaelvdsvldvv
    rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
    epynatlsihqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
    rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
    aacdprhgryltvatifrgrmsmkevdeqmlniqnknssyfvewipnnvktavcdipprg
    lkmsstfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
    eyqqyqdatadeqgefeeeegedea
    

    Sequence, based on observed residues (ATOM records): (download)
    >4lnuB (B:)
    mreivhiqagqcgnqigakfwevisdehgidptgsyhgdsdlqlerinvyyneatgnkyv
    prailvdlepgtmdsvrsgpfgqifrpdnfifgqsgagnnwakghytegaelvdsvldvv
    rkesescdclqgfqlthslgggtgsgmgtlliskireeypdrimntfsvmpspkvsdtvv
    epynatlsihqlventdetysidnealydicfrtlklttptygdlnhlvsatmsgvttcl
    rfpgqlnadlrklavnmvpfprlhffmpgfapltsrgsqqyraltvpeltqqmfdsknmm
    aacdprhgryltvatifrgrmsmkevdeqmlniqnknssyfvewipnnvktavcdipprg
    lkmsstfignstaiqelfkriseqftamfrrkaflhwytgegmdemefteaesnmndlvs
    eyqqyqdatad
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >4lnuD (D:)
    mrgshhhhhhgsdlgkklleaaragqddevrilmangadvnatdasgltplhlaatyghl
    eivevllkhgadvnaidimgstplhlaalighleivevllkhgadvnavdtwgdtplhla
    aimghleivevllkhgadvnaqdkfgktafdisidngnedlaeilqkln
    

    Sequence, based on observed residues (ATOM records): (download)
    >4lnuD (D:)
    dlgkklleaaragqddevrilmangadvnatdasgltplhlaatyghleivevllkhgad
    vnaidimgstplhlaalighleivevllkhgadvnavdtwgdtplhlaaimghleivevl
    lkhgadvnaqdkfgktafdisidngnedlaeilqkln
    

  • Chain 'K':
    No sequence available.