PDB entry 4kar

View 4kar on RCSB PDB site
Description: Crystal structure of FDTS (TM0449) mutant (H53D) with FAD
Class: transferase
Keywords: thyX, FDTS, H53D mutant, FAD, Novel FDTS fold, Convertion of dUMP to dTMP using Tetrahydrofolate, and NAD(P)H, TRANSFERASE
Deposited on 2013-04-22, released 2014-03-26
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-03-26, with a file datestamp of 2014-03-21.
Experiment type: XRAY
Resolution: 2.03 Å
R-factor: 0.198
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (10-11)
      • engineered mutation (64)
    Domains in SCOPe 2.06: d4kara1, d4kara2
  • Chain 'B':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-231)
      • expression tag (11)
      • engineered mutation (64)
    Domains in SCOPe 2.06: d4karb1, d4karb2
  • Chain 'C':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-231)
      • expression tag (11)
      • engineered mutation (64)
    Domains in SCOPe 2.06: d4karc1, d4karc2
  • Chain 'D':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (64)
    Domains in SCOPe 2.06: d4kard_
  • Heterogens: FAD, 2PE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4karA (A:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4karA (A:)
hhmkidildkgfvelvdvmgndlsavraarvsfdmdeerdrhlieylmkhgdetpfehiv
ftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervtek
iseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqw
eiqqyalaiarifkekcpwtfeaflkyaykgdil

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4karB (B:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4karB (B:)
hmkidildkgfvelvdvmgndlsavraarvsfeerdrhlieylmkhgdetpfehivftfh
vkapifvarqwfrhriasynelsgrysyefyipsperlegykttippervtekiseivdk
ayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqweiqqyal
aiarifkekcpwtfeaflkyaykgdilkevqv

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4karC (C:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4karC (C:)
hmkidildkgfvelvdvmgndlsavraarvsfdkdeerdrhlieylmkhgdetpfehivf
tfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervteki
seivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwe
iqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4karD (D:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4karD (D:)
mkidildkgfvelvdvmgndlsavraarvsfddeerdrhlieylmkhgdetpfehivftf
hvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervtekise
ivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqweiq
qyalaiarifkekcpwtfeaflkyaykgdilkev