PDB entry 4eg1

View 4eg1 on RCSB PDB site
Description: Trypanosoma brucei methionyl-tRNA synthetase in complex with substrate Methionine
Class: ligase
Keywords: aminoacyl-tRNA synthetase, aaRS, MetRS, parasite, ligase, protein-inhibitor complex, Rossmann-fold, translation, nucleotide binding, Rossmann Fold, tRNA binding ATP binding
Deposited on 2012-03-30, released 2012-09-12
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-11-15, with a file datestamp of 2017-11-10.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Methionyl-tRNA synthetase, putative
    Species: Trypanosoma brucei brucei [TaxId:999953]
    Gene: Tb10.70.6470
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q38C91
      • conflict (77)
      • engineered mutation (220-222)
      • conflict (267)
      • conflict (271)
    Domains in SCOPe 2.08: d4eg1a1, d4eg1a2
  • Chain 'B':
    Compound: Methionyl-tRNA synthetase, putative
    Species: Trypanosoma brucei brucei [TaxId:999953]
    Gene: Tb10.70.6470
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q38C91 (5-End)
      • expression tag (0-4)
      • conflict (77)
      • engineered mutation (220-222)
      • conflict (267)
      • conflict (271)
    Domains in SCOPe 2.08: d4eg1b1, d4eg1b2, d4eg1b3
  • Heterogens: GOL, MET, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4eg1A (A:)
gpgsmkvekvffvtspiyyvnaaphighvystlitdvigryhrvkgervfaltgtdehgq
kvaeaakqkqvspydfttavagefkkcfeqmdysidyfirttneqhkavvkelwtkleqk
gdiylgryegwysisdesfltpqnitdgvdkdgnpckvslesghvvtwvseenymfrlsa
frerllewyhanpgcivpefrrreviravekglpdlsvsraratlhnwaipvpgnpdhcv
yvwldaltnyltgsrlrvdesgkevslvddfnelerfpadvhvigkdilkfhaiywpafl
lsaglplpkkivahgwwtkdrkkiskslgnvfdpvekaeefgydalkyfllresgfsddg
dysdknmiarlngeladtlgnlvmrctsakinvngewpspaayteedesliqlikdlpgt
adhyylipdiqkaiiavfdvlrainayvtdmapwklvktdperlrtvlyitlegvrvttl
llspilprksvvifdmlgvpevhrkgienfefgavppgtrlgpavegevlfskrstentk
st

    Sequence, based on observed residues (ATOM records): (download) 
    >4eg1A (A:)
ekvffvtspiyyvnaaphighvystlitdvigryhrvkgervfaltgtdehgqkvaeaak
qkqvspydfttavagefkkcfeqmdysidyfirttneqhkavvkelwtkleqkgdiylgr
yegwysisdesfltpqnitdgvdkdgnpckvslesghvvtwvseenymfrlsafrerlle
wyhanpgcivpefrrreviravekglpdlsvsraratlhnwaipvpgnpdhcvyvwldal
tnyltgsrlrvdesgkevslvddfnelerfpadvhvigkdilkfhaiywpafllsaglpl
pkkivahgwwtkdrkkiskslgnvfdpvekaeefgydalkyfllresgfsddgdysdknm
iarlngeladtlgnlvmrctsakinvngewpspaayteedesliqlikdlpgtadhyyli
pdiqkaiiavfdvlrainayvtdmapwklvktdperlrtvlyitlegvrvttlllspilp
rksvvifdmlgvpevhrkgienfefgavppgtrlgpavegevlfskrst

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4eg1B (B:)
gpgsmkvekvffvtspiyyvnaaphighvystlitdvigryhrvkgervfaltgtdehgq
kvaeaakqkqvspydfttavagefkkcfeqmdysidyfirttneqhkavvkelwtkleqk
gdiylgryegwysisdesfltpqnitdgvdkdgnpckvslesghvvtwvseenymfrlsa
frerllewyhanpgcivpefrrreviravekglpdlsvsraratlhnwaipvpgnpdhcv
yvwldaltnyltgsrlrvdesgkevslvddfnelerfpadvhvigkdilkfhaiywpafl
lsaglplpkkivahgwwtkdrkkiskslgnvfdpvekaeefgydalkyfllresgfsddg
dysdknmiarlngeladtlgnlvmrctsakinvngewpspaayteedesliqlikdlpgt
adhyylipdiqkaiiavfdvlrainayvtdmapwklvktdperlrtvlyitlegvrvttl
llspilprksvvifdmlgvpevhrkgienfefgavppgtrlgpavegevlfskrstentk
st

    Sequence, based on observed residues (ATOM records): (download) 
    >4eg1B (B:)
gpgsmkvekvffvtspiyyvnaaphighvystlitdvigryhrvkgervfaltgtdehgq
kvaeaakqkqvspydfttavagefkkcfeqmdysidyfirttneqhkavvkelwtkleqk
gdiylgryegwysisdesfltpqnitdgvdpckvslesghvvtwvseenymfrlsafrer
llewyhanpgcivpefrrreviravekglpdlsvsraratlhnwaipvpgnpdhcvyvwl
daltnyltgsrlrvdesgkevslvddfnelerfpadvhvigkdilkfhaiywpafllsag
lplpkkivahgwwtkdrkkiskslgnvfdpvekaeefgydalkyfllresgfsddgdysd
knmiarlngeladtlgnlvmrctsakinvngewpspaayteedesliqlikdlpgtadhy
ylipdiqkaiiavfdvlrainayvtdmapwklvktdperlrtvlyitlegvrvttlllsp
ilprksvvifdmlgvpevhrkgienfefgavppgtrlgpavegevlfskrste