PDB entry 3sm4

View 3sm4 on RCSB PDB site
Description: Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with a 5'-Phosphorylated 14-mer/12-mer Duplex and Magnesium
Class: hydrolase/DNA
Keywords: homologous recombination, DNA repair, recombineering, single-strand annealing, Type II Restriction Endonuclease Fold, 5'-3' dsDNA exonuclease, HYDROLASE-DNA complex
Deposited on 2011-06-27, released 2011-07-20
The last revision prior to the SCOPe 2.05 freeze date was dated 2011-08-03, with a file datestamp of 2011-07-29.
Experiment type: XRAY
Resolution: 1.88 Å
R-factor: 0.161
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Exonuclease
    Species: Enterobacteria phage lambda [TaxId:10710]
    Gene: exo, Lambda Exonuclease, red-alpha, redX
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03697 (Start-228)
      • engineered mutation (133)
    Domains in SCOPe 2.05: d3sm4a_
  • Chain 'B':
    Compound: Exonuclease
    Species: Enterobacteria phage lambda [TaxId:10710]
    Gene: exo, Lambda Exonuclease, red-alpha, redX
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03697 (3-228)
      • expression tag (1-2)
      • engineered mutation (133)
    Domains in SCOPe 2.05: d3sm4b_
  • Chain 'C':
    Compound: Exonuclease
    Species: Enterobacteria phage lambda [TaxId:10710]
    Gene: exo, Lambda Exonuclease, red-alpha, redX
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03697 (3-228)
      • expression tag (0-2)
      • engineered mutation (133)
    Domains in SCOPe 2.05: d3sm4c_
  • Chain 'D':
    Compound: 5'-d(*tp*cp*gp*gp*tp*ap*cp*ap*gp*tp*ap*g)-3'
  • Chain 'E':
    Compound: 5'-d(p*ap*gp*cp*tp*ap*cp*tp*gp*tp*ap*cp*cp*gp*a)-3'
  • Heterogens: PO4, CL, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3sm4A (A:)
gshmtpdiilqrtgidvraveqgddawhklrlgvitasevhnviakprsgkkwpdmkmsy
fhtllaevctgvapevnakalawgkqyendartlfeftsgvnvtespiiyrdesmrtacs
pdglcsdgnglelacpftsrdfmkfrlggfeaiksaymaqvqysmwvtrknawyfanydp
rmkreglhyvvierdekymasfdeivpefiekmdealaeigfvfgeqwr

    Sequence, based on observed residues (ATOM records): (download) 
    >3sm4A (A:)
tpdiilqrtgidvraveqgddawhklrlgvitasevhnviakprsgkkwpdmkmsyfhtl
laevctgvapevnakalawgkqyendartlfeftsgvnvtespiiyrdesmrtacspdgl
csdgnglelacpftsrdfmkfrlggfeaiksaymaqvqysmwvtrknawyfanydprmkr
eglhyvvierdekymasfdeivpefiekmdealaeigfvfgeqwr

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3sm4B (B:)
gshmtpdiilqrtgidvraveqgddawhklrlgvitasevhnviakprsgkkwpdmkmsy
fhtllaevctgvapevnakalawgkqyendartlfeftsgvnvtespiiyrdesmrtacs
pdglcsdgnglelacpftsrdfmkfrlggfeaiksaymaqvqysmwvtrknawyfanydp
rmkreglhyvvierdekymasfdeivpefiekmdealaeigfvfgeqwr

    Sequence, based on observed residues (ATOM records): (download) 
    >3sm4B (B:)
shmtpdiilqrtgidvraveqgddawhklrlgvitasevhnviakprsgkkwpdmkmsyf
htllaevctgvapevnakalawgkqyendartlfeftsgvnvtespiiyrdesmrtacsp
dglcsdgnglelacpftsrdfmkfrlggfeaiksaymaqvqysmwvtrknawyfanydpr
mkreglhyvvierdekymasfdeivpefiekmdealaeigfvfgeqwr

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3sm4C (C:)
gshmtpdiilqrtgidvraveqgddawhklrlgvitasevhnviakprsgkkwpdmkmsy
fhtllaevctgvapevnakalawgkqyendartlfeftsgvnvtespiiyrdesmrtacs
pdglcsdgnglelacpftsrdfmkfrlggfeaiksaymaqvqysmwvtrknawyfanydp
rmkreglhyvvierdekymasfdeivpefiekmdealaeigfvfgeqwr

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.