PDB entry 3q8g

View 3q8g on RCSB PDB site
Description: Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution
Class: signaling protein
Keywords: CRAL-TRIO, string motif, signaling protein, directed evolution, PIPT, SEC14, phospholipid transporter, lipid, phosphatidylinositol, phosphatidylcholine
Deposited on 2011-01-06, released 2011-02-23
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-10-29, with a file datestamp of 2014-10-24.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.194
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRAL-TRIO domain-containing protein YKL091C
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Gene: YKL091C
    Database cross-references and differences (RAF-indexed):
    • Uniprot P33324
      • engineered mutation (135)
    Domains in SCOPe 2.07: d3q8ga1, d3q8ga2
  • Heterogens: PEE, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3q8gA (A:)
mghhhhhhhhmttsildtypqicspnalpgtpgnltkeqeeallqfrsilleknykerld
dstllrflrarkfdinasvemfveterwreeygantiiedyennkeaedkeriklakmyp
qyyhhvdkdgrplyfaelgginlkkmykittekqmlrnlvkeyelfatyrvpacsrragy
lietsctvldlkgislsnayhvlsyikdvadisqnyypermgkfyiihspfgfstmfkmv
kpfldpvtvskifilgssykkellkqipienlpvkyggtsvlhnpndkfyysdigpwrdp
ryigpegeipnifgkftvts

    Sequence, based on observed residues (ATOM records): (download) 
    >3q8gA (A:)
sildtypqicspnalpgtpgnltkeqeeallqfrsilleknykerlddstllrflrarkf
dinasvemfveterwreeygantiiedyennkeaedkeriklakmypqyyhhvdkdgrpl
yfaelgginlkkmykittekqmlrnlvkeyelfatyrvpacsrragylietsctvldlkg
islsnayhvlsyikdvadisqnyypermgkfyiihspfgfstmfkmvkpfldpvtvskif
ilgssykkellkqipienlpvkyggtsvlhnpndkfyysdigpwrdpryigpegeipnif
gkftvt