PDB entry 3py8

View 3py8 on RCSB PDB site
Description: Crystal structure of a mutant of the large fragment of DNA polymerase I from thermus aquaticus in a closed ternary complex with DNA and ddCTP
Class: Transferase/DNA
Keywords: Modified Klenow fragment, Transferase, polymerase, nucleoside binding, nucleic acid binding, DNA binding, catalytic activity, DNA-directed DNA polymerase activity, 5'-3' exonuclease activity, nucleotides, modified nucleotide, artificial nucleotide, nucleotide probes, Transferase-DNA complex
Deposited on 2010-12-12, released 2011-06-15
The last revision prior to the SCOPe 2.06 freeze date was dated 2011-07-13, with a file datestamp of 2011-07-08.
Experiment type: XRAY
Resolution: 1.74 Å
R-factor: 0.171
AEROSPACI score: 0.57 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase I
    Species: Thermus aquaticus [TaxId:271]
    Gene: polA, pol1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P19821 (Start-539)
      • engineered mutation (321)
      • engineered mutation (454)
    Domains in SCOPe 2.06: d3py8a1, d3py8a2
  • Chain 'B':
    Compound: DNA (5'-d(*gp*ap*cp*cp*ap*cp*gp*gp*cp*gp*cp*(doc))-3')
  • Chain 'C':
    Compound: DNA (5'-d(*ap*a*ap*gp*gp*gp*cp*gp*cp*cp*gp*tp*gp*gp*tp*c)-3')
  • Heterogens: DCT, GOL, MN, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3py8A (A:)
aleeapwpppegafvgfvlsrkepmwadllalaaarggrvhrapepykalrdlkeargll
akdlsvlalreglglppgddpmllaylldpsnttpegvarryggewteeageraalserl
fanlwgrlegeerllwlyreverplsavlahmeatgvrldvaylralslevaeeiarlea
evfrlaghpfnlnsrdqlervlfdelglpaigktektgkrstsaavlealreahpiveki
lqyreltklkstyidplpdlihprtgrlhtrfnqtatatgrlsssdpnlqnipvrtplgq
rirrafiaeegwllvaldysqkelrvlahlsgdenlirvfqegrdihtetaswmfgvpre
avdplmrraaktinfgvlygmsahrlsqelaipyeeaqafieryfqsfpkvrawiektle
egrrrgyvetlfgrrryvpdlearvksvreaaerkafnmpvqgtaadlmklamvklfprl
eemgarmllqvhdelvleapkeraeavarlakevmegvyplavplevevgigedwlsake

    Sequence, based on observed residues (ATOM records): (download) 
    >3py8A (A:)
leeapwpppegafvgfvlsrkepmwadllalaaarggrvhrapepykalrdlkearglla
kdlsvlalreglglppgddpmllaylldpsnttpegvarryggewteeageraalserlf
anlwgrlegeerllwlyreverplsavlahmeatgvrldvaylralslevaeeiarleae
vfrlaghpfnlnsrdqlervlfdelglpaigktektgkrstsaavlealreahpivekil
qyreltklkstyidplpdlihprtgrlhtrfnqtatatgrlsssdpnlqnipvrtplgqr
irrafiaeegwllvaldysqkelrvlahlsgdenlirvfqegrdihtetaswmfgvprea
vdplmrraaktinfgvlygmsahrlsqelaipyeeaqafieryfqsfpkvrawiektlee
grrrgyvetlfgrrryvpdlearvksvreaaerkafnmpvqgtaadlmklamvklfprle
emgarmllqvhdelvleapkeraeavarlakevmegvyplavplevevgigedwlsake

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.