PDB entry 3lcw

View 3lcw on RCSB PDB site
Description: L-KDO aldolase complexed with hydroxypyruvate
Class: lyase
Keywords: Tim barrel, Carbohydrate metabolism, Lyase, Schiff base
Deposited on 2010-01-12, released 2011-01-19
The last revision prior to the SCOPe 2.03 freeze date was dated 2011-01-19, with a file datestamp of 2011-01-14.
Experiment type: XRAY
Resolution: 2.35 Å
R-factor: 0.236
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:83333]
    Gene: b3225, JW3194, nanA, npl
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4
      • engineered mutation (81)
      • engineered mutation (119)
      • engineered mutation (136)
      • engineered mutation (171)
      • engineered mutation (174)
      • engineered mutation (272)
      • engineered mutation (286)
      • engineered mutation (302)
    Domains in SCOPe 2.03: d3lcwa_
  • Chain 'B':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:83333]
    Gene: b3225, JW3194, nanA, npl
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4
      • engineered mutation (81)
      • engineered mutation (119)
      • engineered mutation (136)
      • engineered mutation (171)
      • engineered mutation (174)
      • engineered mutation (272)
      • engineered mutation (286)
      • engineered mutation (302)
    Domains in SCOPe 2.03: d3lcwb_
  • Chain 'C':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:83333]
    Gene: b3225, JW3194, nanA, npl
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4
      • engineered mutation (81)
      • engineered mutation (119)
      • engineered mutation (136)
      • engineered mutation (171)
      • engineered mutation (174)
      • engineered mutation (272)
      • engineered mutation (286)
      • engineered mutation (302)
    Domains in SCOPe 2.03: d3lcwc_
  • Chain 'D':
    Compound: n-acetylneuraminate lyase
    Species: Escherichia coli [TaxId:83333]
    Gene: b3225, JW3194, nanA, npl
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4
      • engineered mutation (81)
      • engineered mutation (119)
      • engineered mutation (136)
      • engineered mutation (171)
      • engineered mutation (174)
      • engineered mutation (272)
      • engineered mutation (286)
      • engineered mutation (302)
    Domains in SCOPe 2.03: d3lcwd_
  • Heterogens: 3PY, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3lcwA (A:)
mghhhhhhhhhhssghiegrhmmatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqg
idglyvggstgeafvqslseraqvleivaeeakgkikliahvgcvstaesqqlaasakrh
gfdavsavtpfyypfsleehcdhyraiidsadglpmvvynipalsgvkltlgqiytlvtl
pgvgalkqtsgdlyqmeqirrehpdlvlyngydeifasgllagadggigstynimgwryq
givkalkegdiqtaqklqtecnkvidlliktgifrglktvlhymdvisvplcrkpfgpvd
ekclpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >3lcwA (A:)
nlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslseraqvl
eivaeeakgkikliahvgcvstaesqqlaasakrhgfdavsavtpfyypfsleehcdhyr
aiidsadglpmvvynipalsgvkltlgqiytlvtlpgvgalkqtsgdlyqmeqirrehpd
lvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkvi
dlliktgifrglktvlhymdvisvplcrkpfgpvdekclpelkalaqqlmq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3lcwB (B:)
mghhhhhhhhhhssghiegrhmmatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqg
idglyvggstgeafvqslseraqvleivaeeakgkikliahvgcvstaesqqlaasakrh
gfdavsavtpfyypfsleehcdhyraiidsadglpmvvynipalsgvkltlgqiytlvtl
pgvgalkqtsgdlyqmeqirrehpdlvlyngydeifasgllagadggigstynimgwryq
givkalkegdiqtaqklqtecnkvidlliktgifrglktvlhymdvisvplcrkpfgpvd
ekclpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >3lcwB (B:)
nlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslseraqvl
eivaeeakgkikliahvgcvstaesqqlaasakrhgfdavsavtpfyypfsleehcdhyr
aiidsadglpmvvynipalsgvkltlgqiytlvtlpgvgalkqtsgdlyqmeqirrehpd
lvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkvi
dlliktgifrglktvlhymdvisvplcrkpfgpvdekclpelkalaqqlmq

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3lcwC (C:)
mghhhhhhhhhhssghiegrhmmatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqg
idglyvggstgeafvqslseraqvleivaeeakgkikliahvgcvstaesqqlaasakrh
gfdavsavtpfyypfsleehcdhyraiidsadglpmvvynipalsgvkltlgqiytlvtl
pgvgalkqtsgdlyqmeqirrehpdlvlyngydeifasgllagadggigstynimgwryq
givkalkegdiqtaqklqtecnkvidlliktgifrglktvlhymdvisvplcrkpfgpvd
ekclpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >3lcwC (C:)
nlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslseraqvl
eivaeeakgkikliahvgcvstaesqqlaasakrhgfdavsavtpfyypfsleehcdhyr
aiidsadglpmvvynipalsgvkltlgqiytlvtlpgvgalkqtsgdlyqmeqirrehpd
lvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkvi
dlliktgifrglktvlhymdvisvplcrkpfgpvdekclpelkalaqqlmq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3lcwD (D:)
mghhhhhhhhhhssghiegrhmmatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqg
idglyvggstgeafvqslseraqvleivaeeakgkikliahvgcvstaesqqlaasakrh
gfdavsavtpfyypfsleehcdhyraiidsadglpmvvynipalsgvkltlgqiytlvtl
pgvgalkqtsgdlyqmeqirrehpdlvlyngydeifasgllagadggigstynimgwryq
givkalkegdiqtaqklqtecnkvidlliktgifrglktvlhymdvisvplcrkpfgpvd
ekclpelkalaqqlmqerg

    Sequence, based on observed residues (ATOM records): (download) 
    >3lcwD (D:)
nlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslseraqvl
eivaeeakgkikliahvgcvstaesqqlaasakrhgfdavsavtpfyypfsleehcdhyr
aiidsadglpmvvynipalsgvkltlgqiytlvtlpgvgalkqtsgdlyqmeqirrehpd
lvlyngydeifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnkvi
dlliktgifrglktvlhymdvisvplcrkpfgpvdekclpelkalaqqlmq