PDB entry 3ifc

View 3ifc on RCSB PDB site
Description: Human muscle fructose-1,6-bisphosphatase E69Q mutant in complex with AMP and alpha fructose-6-phosphate
Class: hydrolase
Keywords: gluconeogenesis, glyconeogenesis, muscle fructose-1,6-bisphosphatase, protein engineering, calcium inhibition, Allosteric enzyme, Carbohydrate metabolism, Hydrolase, Magnesium, Metal-binding, Phosphoprotein
Deposited on 2009-07-24, released 2010-08-11
The last revision prior to the SCOPe 2.06 freeze date was dated 2013-03-13, with a file datestamp of 2013-03-08.
Experiment type: XRAY
Resolution: 1.97 Å
R-factor: 0.167
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.06: d3ifca_
  • Chain 'B':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.06: d3ifcb_
  • Chain 'C':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • engineered mutation (68)
      • see remark 999 (84)
    Domains in SCOPe 2.06: d3ifcc_
  • Chain 'D':
    Compound: Fructose-1,6-bisphosphatase isozyme 2
    Species: Homo sapiens [TaxId:9606]
    Gene: 8789
    Database cross-references and differences (RAF-indexed):
    • Uniprot O00757
      • see remark 999 (84)
    Domains in SCOPe 2.06: d3ifcd_
  • Heterogens: AMP, P6P, SO4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ifcA (A:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifcA (A:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagsqvkkld
vlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasigt
ifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefv
lvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhr
tlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrv
plilgspedvqeyltcvqknqag

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ifcB (B:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifcB (B:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagqvkkldv
lsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasigti
faiyrktsdepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefvlv
ekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhrtl
vyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrvpl
ilgspedvqeyltcvqknqa

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ifcC (C:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifcC (C:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagsqvkkld
vlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasigt
ifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefv
lvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhr
tlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrv
plilgspedvqeyltcvqknq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3ifcD (D:)
tdrspfetdmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygia
gsvnvtgdqvkkldvlsnslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpl
dgssnidclasigtifaiyrktsedepsekdalqcgrnivaagyalygsatlvalstgqg
vdlfmldpalgefvlvekdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapy
garyvgsmvadvhrtlvyggiflypanqkspkgklrllyecnpvayiieqagglattgtq
pvldvkpeaihqrvplilgspedvqeyltcvqknqags

    Sequence, based on observed residues (ATOM records): (download) 
    >3ifcD (D:)
dmltltryvmekgrqakgtgeltqllnsmltaikaissavrkaglahlygiagvkkldvl
snslvinmlqssystcvlvseenkdaiitakekrgkyvvcfdpldgssnidclasigtif
aiyrktsdepsekdalqcgrnivaagyalygsatlvalstgqgvdlfmldpalgefvlve
kdvkikkkgkiyslnegyakyfdaatteyvqkkkfpedgsapygaryvgsmvadvhrtlv
yggiflypanqkspkgklrllyecnpvayiieqagglattgtqpvldvkpeaihqrvpli
lgspedvqeyltcvqknqa