PDB entry 3hea

View 3hea on RCSB PDB site
Description: The L29P/L124I mutation of Pseudomonas fluorescens esterase
Class: hydrolase
Keywords: alpha/beta hydrolase, esterase, covalent adduct, tetrahedral intermediate, Hydrolase, Oxidoreductase, Peroxidase
Deposited on 2009-05-08, released 2010-03-23
The last revision prior to the SCOPe 2.02 freeze date was dated 2010-03-23, with a file datestamp of 2010-03-19.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: 0.191
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3heaa_
  • Chain 'B':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3heab_
  • Chain 'C':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3heac_
  • Chain 'D':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3head_
  • Chain 'E':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3heae_
  • Chain 'F':
    Compound: Arylesterase
    Species: Pseudomonas fluorescens [TaxId:294]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22862 (0-270)
      • engineered (28)
      • engineered (123)
    Domains in SCOPe 2.02: d3heaf_
  • Heterogens: GOL, SO4, EEE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaA (A:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaB (B:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaC (C:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaD (D:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaE (E:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3heaF (F:)
stfvakdgtqiyfkdwgsgkpvlfshgwpldadmweyqmeylssrgyrtiafdrrgfgrs
dqpwtgndydtfaddiaqliehldlkevtlvgfsmgggdvaryiarhgsarvaglvllga
vtpifgqkpdypqgvpldvfarfktellkdraqfisdfnapfyginkgqvvsqgvqtqtl
qiallaslkatvdcvtafaetdfrpdmakidvptlvihgdgdqivpfettgkvaaelikg
aelkvykdaphgfavthaqqlnedllaflkr