PDB entry 3fsl

View 3fsl on RCSB PDB site
Description: Crystal structure of tyrosine aminotransferase tripple mutant (P181Q,R183G,A321K) from Escherichia coli at 2.35 A resolution
Class: transferase
Keywords: Tyrosine aminotransferase, pyridoxal phosphate, internal aldimine, Schiff base, Amino-acid biosynthesis, Aminotransferase, Aromatic amino acid biosynthesis, Transferase
Deposited on 2009-01-09, released 2009-01-27
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-08.
Experiment type: XRAY
Resolution: 2.35 Å
R-factor: 0.215
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fsla_
  • Chain 'B':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fslb_
  • Chain 'C':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fslc_
  • Chain 'D':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fsld_
  • Chain 'E':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fsle_
  • Chain 'F':
    Compound: Aromatic-amino-acid aminotransferase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: b4054, JW4014, tat, tyrB
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04693 (0-396)
      • engineered (170)
      • engineered (172)
      • engineered (309)
    Domains in SCOPe 2.08: d3fslf_
  • Heterogens: PLR, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslA (A:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslB (B:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslC (C:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslD (D:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslE (E:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3fslF (F:)
mfqkvdayagdpiltlmerfkedprsdkvnlsiglyynedgiipqlqavaeaearlnaqp
hgaslylpmeglncyrhaiapllfgadhpvlkqqrvatiqtlggsgalkvgadflkryfp
esgvwvsdptwenhvaifagagfevstypwydeatngvrfndllatlktlqagsivllhp
cchnptgadltndqwdavieilkarelipfldiayqgfgagmeedayairaiasaglpal
vsnsfskifslygervgglsvmcedaeaagrvlgqlkatvrrnyssppnfgaqvvaavln
dealkaswlkeveemrtrilamrqelvkvlstempernfdyllnqrgmfsytglsaaqvd
rlreefgvyliasgrmcvaglntanvqrvakafaavm