PDB entry 3eh8

View 3eh8 on RCSB PDB site
Description: Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium
Class: hydrolase/DNA
Keywords: Protein-DNA complex, Endonuclease, Hydrolase, Intron homing, Mitochondrion, mRNA processing, mRNA splicing, Nuclease, HYDROLASE-DNA COMPLEX
Deposited on 2008-09-11, released 2009-01-13
The last revision prior to the SCOPe 2.04 freeze date was dated 2013-06-26, with a file datestamp of 2013-06-21.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.201
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Intron-encoded DNA endonuclease I-AniI
    Species: Emericella nidulans [TaxId:162425]
    Gene: AnCOB Intron Gene
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03880 (2-253)
      • expression tag (0-1)
      • engineered (12)
      • conflict (60)
      • engineered (79)
      • engineered (110)
      • engineered (231)
    Domains in SCOPe 2.04: d3eh8a1, d3eh8a2
  • Chain 'B':
    Compound: 31-mer
  • Chain 'C':
    Compound: 31-mer
  • Chain 'D':
    Compound: Intron-encoded DNA endonuclease I-AniI
    Species: Emericella nidulans [TaxId:162425]
    Gene: AnCOB Intron Gene
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03880 (2-253)
      • expression tag (0-1)
      • engineered (12)
      • conflict (60)
      • engineered (79)
      • engineered (110)
      • engineered (231)
    Domains in SCOPe 2.04: d3eh8d1, d3eh8d2
  • Chain 'E':
    Compound: 31-mer
  • Chain 'F':
    Compound: 31-mer
  • Chain 'G':
    Compound: Intron-encoded DNA endonuclease I-AniI
    Species: Emericella nidulans [TaxId:162425]
    Gene: AnCOB Intron Gene
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03880 (2-253)
      • expression tag (0-1)
      • engineered (12)
      • conflict (60)
      • engineered (79)
      • engineered (110)
      • engineered (231)
    Domains in SCOPe 2.04: d3eh8g1, d3eh8g2
  • Chain 'H':
    Compound: 31-mer
  • Chain 'I':
    Compound: 31-mer
  • Heterogens: CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3eh8A (A:)
gsdltyaylvglyegdgyfsitkkgkyltyelgielsikdvqliykikkilgigivsfrk
rneiemvalrirdknhlkskilpifekypmfsnkqydylrfrnallsgiiyledlpdytr
sdeplnsiesiintsyfsawlvgfieaegcfsvyklnkdddyliasfdiaqrdgdilisa
irkylsfttkvyldktncsklkvtsvrsveniikflqnapvkllgnkklqyklwlkqlrk
isrysekikipsny

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3eh8D (D:)
gsdltyaylvglyegdgyfsitkkgkyltyelgielsikdvqliykikkilgigivsfrk
rneiemvalrirdknhlkskilpifekypmfsnkqydylrfrnallsgiiyledlpdytr
sdeplnsiesiintsyfsawlvgfieaegcfsvyklnkdddyliasfdiaqrdgdilisa
irkylsfttkvyldktncsklkvtsvrsveniikflqnapvkllgnkklqyklwlkqlrk
isrysekikipsny

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3eh8G (G:)
gsdltyaylvglyegdgyfsitkkgkyltyelgielsikdvqliykikkilgigivsfrk
rneiemvalrirdknhlkskilpifekypmfsnkqydylrfrnallsgiiyledlpdytr
sdeplnsiesiintsyfsawlvgfieaegcfsvyklnkdddyliasfdiaqrdgdilisa
irkylsfttkvyldktncsklkvtsvrsveniikflqnapvkllgnkklqyklwlkqlrk
isrysekikipsny

  • Chain 'H':
    No sequence available.

  • Chain 'I':
    No sequence available.